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中文名称: | G12D KRAS (1-166), Biotinylated at the C-terminus | 品牌: | Amid Biosciences |
英文名称: | G12D KRAS (1-166), Biotinylated at the C-terminus | 货号: | KRAS12D-B-C-301 |
规格: | 100ug | 说明书: | 无 |
货期: | 4周 | 保存温度: | -20℃ |
运输温度: | -20℃ | 来源: | |
氨基酸序列: | 分子量: | ||
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溶解度: | 纯度: | ||
稳定性: | 生物活性: | ||
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Recombinant KRAS G12D protein with the C-terminal AviTag™ peptide and His-tag is produced in E. coli cells and site-specifically biotinylated at AviTag with BirA enzyme (https://amidbiosciences.com/collections/proteins/products/bira-biotin-ligase).
KRAS G12D protein (amino acids 1 – 166; G12D mutant variant of isoform KRAS4B (UNIPROT: P01116-2))
Tag(s): C-terminal AviTag and C-terminal 6xHis tag
MW: 21.9 kDa
Catalog # KRAS12D-B-C-301
Storage buffer: 20 mM HEPES, pH 7.5, 100 mM NaCl, 1 mM MgCl2, 1 mM 2-mercapthoethanol, 10% glycerol.
Purity: >90% by Coomassie staining
Storage is recommended at -70°C/-80°C for longer periods of time. Minimize freeze/thaw cycles. Store on ice before use. The remaining, undiluted protein should be snap frozen in liquid nitrogen.