G12D KRAS (1-166), Biotinylated at the C-terminus

Recombinant KRAS G12D protein with the C-terminal AviTag™ peptide and His-tag is produced in E. coli cells and site-specifically biotinylated at AviTag with BirA enzyme (https://amidbiosciences.com/collections/proteins/products/bira-biotin-ligase).

KRAS G12D protein (amino acids 1 – 166; G12D mutant variant of isoform KRAS4B  (UNIPROT: P01116-2))

Tag(s): C-terminal AviTag and C-terminal 6xHis tag

MW: 21.9 kDa

Catalog # KRAS12D-B-C-301

Storage buffer: 20 mM HEPES, pH 7.5, 100 mM NaCl, 1 mM MgCl₂, 1 mM 2-mercapthoethanol, 10% glycerol. 
Purity: >90% by Coomassie staining 

Storage is recommended at -70°C/-80°C for longer periods of time. Minimize freeze/thaw cycles.  Store on ice before use. The remaining, undiluted protein should be snap frozen in liquid nitrogen.