Mouse Anti citrullinated COL2A1 Antibody (ACC1)

Description
Affinity purified mouse monoclonal anti collagen II (COL2A1) antibody clone ACC1 in PBS, sterile filtered (0.2 µm). The antibody binds to the citrullinated triple helical collagen type II (CII) epitope (position 359-369; ARGLTGRPGDA)¹, the C1 epitope. The ACC1 antibody recognizes the second citrulline at position 365 in mouse CII¹. The antibody binds to flexible triple-helical CII determinants as well as many citrullinated CII peptides and was found to cross-react with several non-citrullinated epitopes on native CII². The antibody has been used in applications including ELISA^1,2, immuno­histochemistry (IHC)¹ and bead-based multiplex immunoassays².

Target with alternative names: COL2A1, Collagen alpha-1(II) chain, CII, Col II, citrullinated CII; citrullinated Col II; citrullinated proteins. UniProt: P28481.
Immunogen: Bovine Collagen type II ¹
Species reactivity: Mouse, human
Isotype:  Mouse IgG2a, κ
Specificity: ACC1 antibody binds the citrullinated C1 epitope. The ACC1 antibody cross-reacts with triple-helical epitopes on CII, but not with other collagens or the proteins tested in Ge et al², except for a few citrullinated peptides.²
Concentration: 1 mg/ml
Supplied in: PBS
Storage: Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -‑20°C long term. Avoid repeated freeze/thaw cycles.
Recommended dilution: It is recommended the user determines the optimal dilution for their application. The typical starting working dilutions are: IHC 1:100, ELISA 1:200 - 1:500.