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3D cell culture Kit
报价：10033.00
SpheroTribe provides a simple toolkit to generate consistent and robust 3D cell structures (spheroids, organoids) without restriction of cell type. Simply dilute the SpheroTribe solution into your culture medium of choice, watch your cells turn into uniformly sized 3D spheroids and collect them for your downstream assays.
Once diluted in your culture medium of choice, our concentrated polymer-based solution increases the medium viscosity favouring cell-cell contacts. SpheroTribe offers a simple method to generate homogeneous 3D cell structures with increased control over their size and shape, which can be easily handled and washed for downstream experiments.
SpheroTribe is particularly useful to boost aggregation when working with challenging cells, minimize variability between samples and improve the consistency of your migration/invasion assays, immunostaining, drug screening or in vivo implantation experiments.
In addition to the SpheroTribe solution, the full kit also includes U-bottom plates and wide-opening tips so you have everything you need to get started with your 3D cell culture experiments.
Storage recommendation for the methylcellulose solution:
4°C for (at least) 10 months.
So far, SpheroTribe has been successfully used for spheroid/organoid formation with the following cell types:
Patient-derived stem-like glioblastoma cells (GB P3, BL13), human glioblastoma cell lines (U87 & T98G), HeLa, human vaginal mucosal melanoma (HMV-II), human primary colorectal cancer cells, human colorectal cancer cells (HT-29 & HCT-116), human breast cancer cells (MDA-MB 231), human induced pluripotent stem cells, monkey kidney fibroblast-like cell line (COS-7), primary neurons from rat embryos (E18) & murine melanoma cells (B16F10).
Experimental assays:
Once spheroids have grown to your desired size, you can use them for any kind of assay according to your regular workflow. The SpheroTribe solution can be readily washed off, leaving a spheroid available for other tests at any stage of your protocol.
Example of in situ assays you can perform directly on the U-bottom plate supplied:
Examples of downstream assays that might require transferring spheroids to other vessels:
For more guidance on downstream assays, check out our FAQ section containing useful tips & example protocols.
Results：
Uniform patient-derived glioblastoma spheroids generated using the SpheroTribe kit and visible by the naked eye after 4 days of culture 
SpheroTribe improves the formation of unique & circular human glioblastoma spheroids 
| 中文名称 | 产品名称 | 货号 | 规格 |
| 3D细胞培养套装 | 3D cell culture Kit | TDA-SPK-KIT | 1Kit(25ml) |
| 3D细胞培养套装 | 3D cell culture Kit | TDA-SPK-MINIKIT | 1Kit(2.5ml) |
| 甲基纤维素培养基 | Methylcellulose | TDA-SPK-2-25 | 1瓶（25ml） |
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