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TMB 1-Component HRP Membrane Substrate (SUBM)
TMB 1-Component HRP Membrane Substrate (SUBM)
- 中文名称：
- TMB 1-Component HRP Membrane Substrate (SUBM)
- 英文名称：
- TMB 1-Component HRP Membrane Substrate (SUBM)
- 品牌：
- Immuno Chemistry
- 品牌介绍：
- ADI(Alpha Diagnostic International) 公司位于美国德克萨斯州圣安东尼奥，具有USDA认证的动物试验设施，主要研发用于人、小鼠、大鼠、猴、豚鼠、地鼠、雪貂、家畜、家禽等20余物种的各类ELISA试剂盒、细胞生物学和免疫研究常用一抗、二抗、抗血清及辅助 试剂，可提供多肽合成、抗体定制服务。
- 货号：
- 6280
- 规格：
- 100 mL
- 保存建议：
- 2-8°C
- 背景资料：
- TMB 1-Component HRP Membrane Substrate (SUBM) is suitable for membrane applications using horseradish peroxidase (HRP) as the conjugated detection enzyme. SUBM should not be used for microwell (ELISA) applications.TMB 1 Component HRP Membrane Substrate is a one-component formulation containing stabilized 3,3’,5,5’-tetramethylbenzidine (TMB). The TMB substrate is oxidized by the peroxidase enzyme to yield an insoluble dark blue reaction product. SUBM is supplied ready-to-use at 1X.Best results are obtained by equilibrating TMB 1-Component HRP Membrane Substrate to room temperature (25°C) prior to use. After probing with the antibody and HRP reagents, wash membrane thoroughly and transfer the membrane into a clean container. Cover the membrane surface with ample amount of SUBM and incubate 5-20 minutes. The substrate will react with sites on the membrane containing peroxidase, producing an insoluble permanent dark blue reaction product.For best results, monitor the substrate color development process until the target protein bands are visible. To stop the reaction, rinse the membrane with reagent quality water. If the reaction proceeds too long, there will be excessive background staining and diminished resolution of the target peptide or protein banding regions. If the color development is too rapid or intense, it is recommended to dilute the antibodies or conjugates or shorten the incubation period.
- 货期：
- 4-6周
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
A ready-to-use formulation containing TMB in a mildly acidic buffer. This TMB substrate is useful for membrane-based immunoblotting applications using HRP-conjugates for detection. Yields an insoluble, permanent dark blue reaction product.
