
ELISA Grade Bovine Type I Collagen, 0.5 mg/ml x 1ml
| 产品名称 | ELISA 级牛 I 型胶原蛋白 ELISA Grade Bovine Type I Collagen |
| 货号 | 1002 |
| 使用说明 | 仅供研究使用 - 不用于人类或治疗用途 For Research Use Only - Not Human or Therapeutic Use |
| 产品描述 | 高度纯化的 I 型胶原蛋白(经 3M 盐酸胍、DEAE-纤维素和 Na2HPO4 处理) Highly purified type I collagen (treated with 3M guanidine, DEAE-cellulose, and Na2HPO4) |
| 应用 | 用作 ELISA 抗原,检测实验动物和人类血清中针对牛 I 型胶原蛋白的特异性抗体,如胶原诱导性关节炎(CIA)模型以及关节炎或其他疾病患者,同时也可用于正常对照,以研究抗胶原蛋白抗体的多样性。 Use as an ELISA antigen to detect antibodies specific to Bovine type I collagen in sera from experimental animals and humans, such as collagen-induced arthritis (CIA) models and patients with arthritis or other diseases, as well as normal controls to study the diversity of anti-collagen antibodies. |
| 规格 | 1 ml |
| 剂型 | 0.05M 乙酸中的 0.5 mg/ml 溶液 0.5 mg/ml solution in 0.05M acetic acid |
| 来源 | 牛皮肤 Bovine skin |
| 分子量 | 300 kDa |
| 纯度 | >99%(SDS-PAGE 法),不含胃蛋白酶和蛋白聚糖 >99% by SDS-PAGE, free of pepsin and proteoglycans |
| 使用方法 | Chondrex, Inc. 建议使用冷的 1X 胶原蛋白稀释缓冲液(10X 胶原蛋白稀释缓冲液,货号 9003,随本产品提供)稀释本产品,以防止胶原蛋白分子聚集。包被板时,取 ELISA 级胶原蛋白等分样品,在使用前用冷的 1X 胶原蛋白稀释缓冲液进行稀释。请勿保存已稀释的胶原蛋白溶液。包被 ELISA 板的最佳胶原蛋白浓度为 5 µg/ml,但可能因检测方法或所用板类型(如细胞培养板)的不同而有所差异。 Chondrex, Inc. recommends diluting this product with cold 1X Collagen Dilution Buffer, (10X Collagen Dilution Buffer, Cat # 9003 as provided with this product), to prevent collagen molecules from aggregating. For plate coating, take aliquots of ELISA grade collagen and dilute them with cold 1X Collagen Dilution Buffer just before use. Do not keep diluted collagen solutions. The optimum collagen concentration to coat ELISA plates is 5 µg/ml, but this may vary based on the assay or type of plate used, such as cell culture plates. |
| 储存条件 | -20°C。本产品在酸性条件下于 4°C 可稳定保存数周;按出厂状态在 -20°C 避光条件下可保存一年,但在中性条件下会逐渐形成聚合胶原蛋白并发生降解。 -20°C. This product is stable in acidic conditions at 4°C for several weeks or at -20°C in the dark for one year as supplied but will gradually form polymeric collagen and degrade under neutral conditions. |
| 稳定性 | 1 year |
| 备注 | N/A |
| 参考文献 | Seyer J. et al. Clin. Invest. 59: 241-248 (1977) Terato K. et al. Arthritis Rheumatism 33: 1493-1500 (1990) Terato K. et al. Clin. Immunol. Immunopathol. 79: 142-154 (1996) |

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