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Tumor Necrosis Factor alpha (TNFa)
[Tumor Necrosis Factor alpha (TNFa)]
- 中文名称：
- Tumor Necrosis Factor alpha (TNFa)
- 英文名称：
- [Tumor Necrosis Factor alpha (TNFa)]
- 品牌：
- MyBioSource
- 品牌介绍：
- MyBioSource 的第一个愿景是成为全球头号高质量试剂的供应商。MyBioSource 公司的目标是提供给研究人员、科学家和客户一站式的所有试剂，无论是单克隆抗体，多克隆抗体，重组蛋白，多肽等。
- 货号：
- MBS6508405
- 规格：
- 1 mg；5x1 mg；
- 保存建议：
- May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.||TNF-a Neutralization Assay:|1. Plate 2 x 10e4 murine L929B cells into individual wells of 96-well (flat bottomed) microtiter plate.|2. Incubate overnight at 37 degree C in humidified 5% CO2 incubator.|3. The next day, prepare serial dilutions of T9160-31 in medium containing 2ug/ml of actinomycin D, for a final volume of 50ul.|4. Add 50ul of mouse TNFa (50pg/ml final concentration) to each antibody dilution and incubate for 1 hour at 37 degree C.|5. Add 100ul of the antibody/antigen mixture to the pre-seeded L929B cells.|6. Incubate 24 hours at 37 degree C, 5% CO2.|7. Score wells for cytotoxicity.
- 货期：
- 6-8周
- 来源宿主：
- Rat
- 反应种属：
- Mouse
- 应用：
- Dot Blot (DB), Neutralizing (NEU), Western Blot (WB)
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 其他：
- 【克隆性】：Monoclonal
【亚型】：IgG1
【克隆号】：[0.T.151]
- 说明书：
