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Antigen-Down Assay Diluent
Antigen-Down Assay Diluent
- 中文名称：
- Antigen-Down Assay Diluent
- 英文名称：
- Antigen-Down Assay Diluent
- 品牌：
- Immuno Chemistry
- 货号：
- 629
- 规格：
- 100 mL
- 保存建议：
- 2-8°C
- 背景资料：
- Minimizes clotting potential and reduces non-specific binding.Antigen-Down Assay Diluent is formulated for testing serum and plasma samples in situations where an undesirable clotting event could occur while performing an antigen-down ELISA. It contains calcium chelating agents and other proprietary additives to eliminate the risk of clotting events in plasma samples and minimize interference from complement and thrombin activity.Antigen-Down Assay Diluent also provides a consistent protein matrix environment that will stabilize the antigen-specific target IgG analyte during high dilution ELISA titration protocols. Assessment of high titer, humoral IgG responses often requires dilutions >100,000-fold. This extensive dilution is often necessary to obtain a chromogen absorbance value that is within the sensitivity detection limits of the ELISA plate reader.This novel assay diluent formulation includes mammalian protein and detergent additives to reduce the non-specific adsorption of generic serum IgG molecules onto the coated and blocked ELISA well surface. This feature can be especially critical when assessing sera derived from human, porcine, or bovine sources, which show a higher tendency to non-specifically adsorb. By minimizing such non-specific binding events, Antigen-Down Assay Diluent inhibits undesirable background noise and increases the sensitivity of the assay.To use, simply add 50-100 µL to every well of the ELISA plate, including all wells designated for standards, controls, and samples. Then add the standards, controls, and samples to the plate. Incorporation of an antimicrobial agent into the formulation allows for room temperature bench-top use and extensive storage stability at 2-8°C.
- 货期：
- 4-6周
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
For use with serum and plasma samples in antigen-down ELISAs. Proprietary additives reduce non-specific binding and minimize clotting potential. Enhances specific signal without denaturing the plate-adsorbed antigen molecules.
