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ELISA Blocking Buffer
ELISA Blocking Buffer
- 中文名称：
- ELISA Blocking Buffer
- 英文名称：
- ELISA Blocking Buffer
- 品牌：
- Immuno Chemistry
- 货号：
- 643
- 规格：
- 1 L
- 保存建议：
- 2-8°C
- 背景资料：
- ELISA Blocking Buffer is designed to avoid false positives associated with animal proteins (e.g., BSA) and eliminate non-specific background noise in antibody-sandwich and antigen-down ELISAs without the use of conventional protein additives. By depositing inert, synthetic blocking molecules onto the plate, ELISA Blocking Buffer reduces non-specific binding of enzyme-labeled conjugates to the microtiter plate, enhancing the sensitivity of the assay. Its synthetic blockers also stabilize coated protein for improved retention of antigen epitope or antibody binding activity during long-term storage. ELISA Blocking Buffer contains an antimicrobial agent for room temperature blocking and long-term storage of dried plates at 2-8°C. ELISA Blocking Buffer works on all types of polystyrene plates except Immulon® II microplates. When blocking with ELISA Blocking Buffer, we recommend Corning® 96-Well EIA/RIA Stripwell™ microplates (ICT catalog #25). When preparing plates, the antibody or antigen is typically coated using 50-200 µL of coating solution per well. After coating, plates are normally washed to remove unbound proteins and then blocked using a larger volume of blocking buffer than was used for coating, such as 300 µL per well. This ensures that all uncoated regions inside the well are blocked. A 96-well plate blocked using this method will require 28.8 mL of blocking solution. Allow approximately 10% extra blocking buffer to account for losses during pipetting.
- 货期：
- 4-6周
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Eliminate interference and nonspecific background associated with antibody-coated ELISA formats and sandwich ELISAs. ELISA Blocking Buffer (formerly known as Synblock) provides low background performance without the use of conventional cross-reactive protein additives.
