-
Green Live/Dead Stain
Green Live/Dead Stain
- 中文名称：
- Green Live/Dead Stain
- 英文名称：
- Green Live/Dead Stain
- 品牌：
- Immuno Chemistry
- 货号：
- 6342
- 规格：
- 50 ul
- 保存建议：
- -20°C
- 背景资料：
- Green Live/Dead Stain is a membrane impermeant fluorescent vital stain for differentiating live and dead cells., , This green Live/Dead Stain is a vital dye that exhibits intact cell membrane exclusion properties analogous to the popular red fluorescing vital dyes, Propidium Iodide (PI), 7-aminoactinomycinD (7-AAD), and DRAQ7™. Like the red fluorescing DNA dyes, Green Live/Dead Stain is excluded from intact, healthy cells due to its polar nature. In the presence of cells exhibiting compromised membrane integrity, Green Live/Dead Stain penetrates the cell and nuclear membrane barriers and intercalates tightly to DNA in a manner analogous to PI and 7-AAD. When bound to DNA, it acquires a greatly enhanced fluorescence potential (>2000X) in the green emission range. These important vital dye properties enable Green Live/Dead Stain to be used in flow cytometry-based protocols and microscopy analysis techniques to assess the percentage of late apoptotic, necrotic, and membrane-compromised cells within a sample cell population. The green fluorescence emission permits new combinations of dyes to be used in the understanding of cell death and apoptosis, such as use with red SR-FLICA® assays, far-red FLICA 660 caspase assays, and other visible range fluor-tagged ligands., , Green Live/Dead Stain only stains nuclei in dead and permeabilized cells. Green Live/Dead Stain does not enter intact, live cells. Cells may be fixed or permeabilized for use of Green Live/Dead Stain as a nuclear counterstain. With negligible toxicity, Green Live/Dead Stain can be used in long-term viability assays to assess cytotoxicity and apoptosis., , For microscopy applications, Green Live/Dead Stain will function as a DNA counterstain for fixed cell and tissue samples in IF, IHC, high content screening, and cell-based assays.
- 货期：
- 4-6周
- 样本类型：
- Cell culture
- 检测方法：
- Flow cytometry, Fluorescence microscope
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Green Live/Dead Stain (catalog #6342) is a vital dye that exhibits intact cell membrane exclusion properties analogous to the popular red fluorescing vital dyes, Propidium Iodide (PI), 7-amino- actinomycin D (7-AAD) and DRAQ7™. Like the red fluorescing dyes, Green Live/Dead Stain is excluded from intact, healthy cells due to its polar nature. In the presence of cells exhibiting compromised membrane integrity, Green Live/Dead Stain penetrates the cell and nuclear membrane barriers and intercalates tightly to DNA in a manner analogous to PI and 7-AAD. When bound to DNA, it acquires a greatly enhanced fluorescence potential (>2000X) in the green emission range. These important vital dye properties enable Green Live/Dead Stain to be used in flow cytometry-based protocols to assess the percentage of late apoptotic, necrotic, and membrane-compromised cells within a sample cell population. When bound to nucleic acids, the maximum absorption of Green Live/Dead Stain is 495 nm and the maximum emission is 512 nm (Figure 1). Cells can be viewed through a fluorescence microscope (Figures 2 & 5) or analyzed with a flow cytometer (Figure 4). Green Live/Dead Stain is provided as a 500 µM concentrated stock solution dissolved in DMSO. For flow cytometry applications, a staining concentration of 50 nM is recommended. Therefore, using sample sizes of 0.5 mL, a single 50 µL vial (catalog #6342) provides enough reagent for 1000 tests. For fluorescence microscopy applications, a usage concentration of 0.5 µM is suggested. In this way, a vial is sufficient for 100 tests (0.45 mL sample sizes). Green Live/ Dead Stain can be used with ICT’s red FLICA® 660 caspase inhibitor reagents (e.g., catalog #9120) to identify four populations of cells: living; early apoptotic; late apoptotic; and necrotic (Figures 4 & 5).
