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DNA Damage ELISA Kit
DNA Damage ELISA Kit
- 中文名称：
- DNA Damage ELISA Kit
- 英文名称：
- DNA Damage ELISA Kit
- 品牌：
- Immuno Chemistry
- 货号：
- 9143
- 规格：
- 96-Well Plate x 1
- 保存建议：
- 8-OHdG at -20°C, other components at 2-8°C
- 背景资料：
- 8-hydroxy-2-deoxyguanosine (8-OHdG) is produced by the oxidative damage of DNA by reactive oxygen and nitrogen species and serves as an established marker od oxidative stress. Hydroxylation of guanosine occurs in response to both normal metabolic processes and a variety of environmental factors (i.e. anything that increases reactive oxygen and nitrogen species). Increased levels of 8-OHdG are associated with the aging process as well a number of pathological conditions including cancer, diabetes, and hypertension.In complex samples such as plasma, cell lysates, and tissues, 8-OHdG can exist either as the free nucleoside or incorporated in DNA. Once the blood enters the kidney, free 8-OHdG is readily filtered into the urine, while larger DNA fragments remain in the bloodstream. Because of the complexity of plasma samples, urine is a more suitable matrix for the measurement of free 8-OHdG than plasma. Urinary levels of 8-OHdG range between 2.7-13 ng/mg creatine, while plasma levels of free 8-OHdG have been reported to be between 4-21 pg/mL as determined by LC/MS.ICT’s DNA Damage (8-OHdG) ELISA is a competitive assay that can be used for the quantification of 8-OHdG in urine, cell culture, plasma, and other sample matrices. The ELISA utilizes an 8-hydroxy-2-deoxyguanosine-coated plate and an HRP-conjugated 8-OHdG specific detection antibody. This produces an assay with a useful range of 0.94 - 60 ng/mL, and a sensitivity of 0.59 ng/mL. Other highlights of this kit include a quick incubation time (60 minutes), stable reagents, and a user-friendly protocol.It is important to note that the 8-OHdG antibody used in this assay recognizes both free 8-OHdG and DNA-incorporated 8-OHdG. Since complex samples such as plasma, cell lysates, and tissues are comprised of mixtures of DNA fragments and free 8-OHdG, concentrations of 8-OHdG reported by ELISA methodology will not coincide with those reported by LC-MS where the single nucleoside is typically measured. This should be kept in mind when analyzing and interpreting experimental results.
- 货期：
- 4-6周
- 样本类型：
- Culture medium, urine, plasma/serum, cell lysates, tissue samples, saliva
- 检测方法：
- Absorbance Plate Reader
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Assess DNA damage by measuring 8-hydroxy-2-deoxyguanosine in urine, cell culture, plasma and other sample matrices. This kit offers a quick incubation time, stable reagents, and a user-friendly protocol. Analyze using an absorbance plate reader.
