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Nitric Oxide Synthase Assay
Nitric Oxide Synthase Assay
- 中文名称：
- Nitric Oxide Synthase Assay
- 英文名称：
- Nitric Oxide Synthase Assay
- 品牌：
- Immuno Chemistry
- 货号：
- 9155
- 规格：
- 50-100 Tests
- 保存建议：
- 2-8°C
- 背景资料：
- Nitric oxide synthases are a family of enzymes capable of catalyzing the production of nitric oxide (NO). NO is an important molecule that is involved in regulating a variety of cellular processes such as angiogenesis, peristalsis, and the immune response to invading pathogens. Reactive nitrogen species (RNS) are a family of antimicrobial molecules derived from NO and superoxide through the enzymatic activities of nitric oxide synthase (NOS).ICT’s Nitric Oxide Synthase Assay provides a good screening option for assessing the potency of nitrosative stress inhibitor and activator reagents, and will help to determine how oxidative and nitrosative stress modulates varied intracellular pathways. This kit assesses the overall intracellular levels of free nitric oxide and NOS using a Diaminofluorescein-2 Diacetate (DAF-2DA) dye.The kit provides all the essential reagents and an easy to follow protocol to assess changes in intracellular NO and NOS levels using flow cytometry, fluorescence plate reader, and fluorescence microscopy. The DAF-2DA dye quickly penetrates membrane structures and accumulates within the cell. Once inside the cell the diacetate groups on the DAF-2DA reagent are hydrolyzed by cytosolic esterases, allowing for the release and sequestration of DAF-2 inside the cell. Production of nitric oxide converts the non-fluorescent DAF-2 dye to its fluorescent triaole derivative, DAF-2T3-7.Nitric Oxide Synthase Assay requires minimal procedural steps and hands-on time to complete. Samples are stained for 1 hour with DAF-2DA dye. Cells are washed to remove excess dye, and then treated experimentally. Since the unbound reagent is non-fluorescent, subsequent wash steps are not required, thus simplifying the assay procedure. Following treatment, cells are ready for analysis by flow cytometry.Each kit will enable the assessment of up to 50 (0.5 mL) or 100 (0.25 mL) samples. For microscopy usage, Hoechst 33342 is included with the kit to concurrently label nuclei after labeling with DAF-2DA. DAF-2DA optimally excites at 488 nm, and has a peak emission at 515 nm. Hoechst 33342 can be seen using a UV-filter with excitation at 365 nm and emission at 480 nm.
- 货期：
- 4-6周
- 样本类型：
- Cell culture
- 检测方法：
- Flow cytometry, Fluorescence microscope, fluorescence plate reader
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Assess the potency of nitrosative stress inhibitor and activator reagents. Measure the overall intracellular levels of free nitric oxide and NOS using a DAF-2DA dye. Analyze on a flow cytometer, fluorescence plate reader, or fluorescence microscope.
