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Anti-Amyloid beta peptide (A-beta 40/42) Antibody (Biotin)
Anti-Amyloid beta peptide (A-beta 40/42) Antibody (Biotin)
- 中文名称：
- Anti-Amyloid beta peptide (A-beta 40/42) Antibody (Biotin)
- 英文名称：
- Anti-Amyloid beta peptide (A-beta 40/42) Antibody (Biotin)
- 品牌：
- Biosensis
- 货号：
- M-1742-50-B
- 规格：
- 50 µg
- 保存建议：
- Spin vial briefly before opening. Reconstitute in 50 µL sterile-filtered, ultrapure water to give a concentration of 1 mg/mL. Centrifuge to remove any insoluble material. Final buffer is PBS, pH 7.4 without preservative.After reconstitution keep aliquots at -20°C to -70°C for a higher stability. At 2-8°C keep up to one week; use sterile methods and pipettes. Highly purified glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles. Keep tightly closed when not in use and protected from light.
- 背景资料：
- The amyloid beta peptide is derived from the cleavage of the Amyloid precursor protein (APP) and varies in length from 39 to 43 amino acids. However, the form(s) of amyloid-beta peptide (Aβ associated with the pathology characteristic of Alzheimer's disease (AD) remains unclear. In particular, the neurotoxicity of intraneuronal Aβ accumulation is an area of considerable research and controversy principally because antibodies thought to be specific for Aβ have been shown to actually detect intraneuronal APP and not Aβ exclusively.
MOAB-2 (mouse IgG2b) is a pan-specific, high-titer antibody to Aβ residues 1-4 as demonstrated by biochemical and immunohistochemical analyses (IHC), and is highly specific just to amyloid beta peptide. MOAB-2 did not detect APP or APP-CTFs in cell culture media/lysates (HEK-APPSwe or HEK APPSwe/BACE1) or in brain homogenates from transgenic mice expressing 5 familial AD (FAD) mutation (5xFAD mice).
Using IHC on 5xFAD brain tissue, MOAB-2 immunoreactivity co-localized with C-terminal antibodies specific for Aβ40 and Aβ42. MOAB-2 did not co-localize with either N- or C-terminal antibodies to APP. In addition, no MOAB-2-immunreactivity was observed in the brains of 5xFAD/BACE-/- mice, although significant amounts of APP were detected by N- and C-terminal antibodies to APP, as well as by 6E10. In both 5xFAD and 3xTg mouse brain tissue, MOAB-2 co-localized with cathepsin-D, a marker for acidic organelles, further evidence for intraneuronal Aβ, distinct from Aβ associated with the cell membrane. MOAB-2 demonstrated strong intraneuronal and extra-cellular immunoreactivity in 5xFAD and 3xTg mouse brain tissues.
Biosensis now offers biotinylated MOAB-2 antibody allowing more flexibility in experimental design by using the biotin-avidin/streptavidin detection method. Biotinylated MOAB-2 antibody may also help to reduce background staining in difficult-to-stain tissues and increase detection sensitivity. The ability of biotinylated MOAB-2 antibody to detect amyloid beta has been validated by IHC.
Purified, non-biotinylated MOAB-2 antibody is available here.
- 货期：
- 4-6周
- 来源宿主：
- Mouse
- 应用：
- ELISA, ICC, IHC-Frozen, IHC-Paraffin-embedded, IP, WB
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
Our Anti-Amyloid beta peptide (A-beta 40/42) mouse monoclonal primary antibody detects human and rat Amyloid beta peptide (A-beta 40/42), and is igg. It is validated for use in ELISA, ICC, IHC-Frozen, IHC-Paraffin-embedded, IP, WB.
