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Fluo-4, AM Ester Fluorescent Calcium Indicator
Fluo-4, AM Ester Fluorescent Calcium Indicator
- 中文名称:
- Fluo-4, AM Ester Fluorescent Calcium Indicator
- 英文名称:
- Fluo-4, AM Ester Fluorescent Calcium Indicator
- 品牌:
- Antibodies Inc
- 品牌介绍:
- Antibodies Inc.成立于1962年, 在神经科学领域备受推崇,拥有针对关键神经科学靶点的一流小鼠单克隆抗体。最初专注于二抗和免疫测定,在2005年,与加州大学戴维斯分校和美国国立卫生研究院合作,成为高度验证的NeuroMab系列小鼠单克隆抗体的商业供应商。这些高品质的抗体经过全部测试,数十种已进行基因敲除验证,并提供四种不同荧光标记物的抗体。我们的定制抗体服务提供量身定制的解决方案,包括单克隆和多克隆选项,以及生产、共轭和免疫测定服务。所有这些工作都在位于加利福尼亚州戴维斯的ISO-13485认证设施中完成。
- 货号:
- 81-3006
- 规格:
- 10 x 50 µg
- 保存建议:
- 在-20°C下储存,使用干燥剂;避光保存。
- 背景资料:
- To study the roles of calcium, a key intracellular messenger in cells, it is essential to quantitatively monitor its concentration. The most widely used method of Ca2+ detection is the use of fluorescent Ca2+ indicators. Fluo-4 is an analog of Fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm that gives higher fluorescence signal. Fluo-4 has its absorption maximum at 494 nm, thus making it excitable by the argon-ion laser. Fluo-4 is essentially nonfluorescent without Ca2+ present, but the fluorescence increases at least 100 times on Ca2+ binding. Also, because Fluo-4 binds Ca2+ more weakly (higher Kd) than do fura-2 and indo-1, it is more useful for measuring high transient Ca2+ concentration during Ca2+ spikes. Fluo-4, AM ester is a membrane-permeant form of Fluo-4 calcium indicator that can enter into into cells via incubation.
- 货期:
- 4-6周
- 来源宿主:
-
- 应用:
- ICC, IHC, EM
- 免责声明:
- *本产品仅供科研实验使用,不得用于临床诊断。*
- 其他:
免疫原物种:
克隆性:N/A
克隆号:
浓度:N/A
分子量:1096.95 Da