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Fura-2, AM Ester Fluorescent Calcium Indicator
Fura-2, AM Ester Fluorescent Calcium Indicator
- 中文名称:
- Fura-2, AM Ester Fluorescent Calcium Indicator
- 英文名称:
- Fura-2, AM Ester Fluorescent Calcium Indicator
- 品牌:
- Antibodies Inc
- 品牌介绍:
- Antibodies Inc.成立于1962年, 在神经科学领域备受推崇,拥有针对关键神经科学靶点的一流小鼠单克隆抗体。最初专注于二抗和免疫测定,在2005年,与加州大学戴维斯分校和美国国立卫生研究院合作,成为高度验证的NeuroMab系列小鼠单克隆抗体的商业供应商。这些高品质的抗体经过全部测试,数十种已进行基因敲除验证,并提供四种不同荧光标记物的抗体。我们的定制抗体服务提供量身定制的解决方案,包括单克隆和多克隆选项,以及生产、共轭和免疫测定服务。所有这些工作都在位于加利福尼亚州戴维斯的ISO-13485认证设施中完成。
- 货号:
- 81-3018
- 规格:
- 10 x 100 µg
- 保存建议:
- 在-20°C下储存,使用干燥剂;避光保存。
- 背景资料:
- To study the roles of calcium, a key intracellular messenger in cells, it is essential to quantitatively monitor its concentration. The most widely used method of Ca2+ detection is the use of fluorescent Ca2+ indicators. Fura-2, AM ester is a ratiometric fluorescent dye that binds to free intracellular calcium, and is the preferred dye for ratiometric imaging microscopy. Fura-2, AM ester is membrane-permeant and thus can be loaded into cells via incubation. Fura-2, AM ester itself does not bind Ca2+, but it is readily hydrolyzed to Fura-2 by endogenous esterases once it enters cells. Fura-2 fluorescence can be detected using flow cytometry, fluorescence microscopy, or certain fluoresence microplate readers. Upon calcium binding, the fluorescent excitation maximum of the indicator undergoes a blue shift from 363 nm (Ca2+-free) to 335 nm (Ca2+-saturated), while the fluorescence emission maximum is relatively unchanged at ~510 nm. A major use of Fura-2 is in drug discovery, for measuring intracellular calcium mobilization after activation of G protein-coupled receptors (GPCRs) and calcium channels.
- 货期:
- 4-6周
- 来源宿主:
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- 应用:
- ICC, IHC, EM
- 免责声明:
- *本产品仅供科研实验使用,不得用于临床诊断。*
- 其他:
免疫原物种:
克隆性:N/A
克隆号:
浓度:N/A
分子量:1001.9 Da