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ROCK Activity Immunoblot Kit
ROCK Activity Immunoblot Kit
- 中文名称：
- ROCK Activity Immunoblot Kit
- 英文名称：
- ROCK Activity Immunoblot Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA44565
- 规格：
- 20 Assays|2x20 Assays|3x20 Assays|4x20 Assays|5x20 Assays
- 保存建议：
- Store active ROCK-II at -80 degree C and all other kit components at -20 degree C until their expiration dates. Avoid multiple freeze/thaw cycles.
- 货期：
- 6-8周
- CAS号：
- 445-65-8
- 纯度：
- N/A
- 产品形式：
- N/A
- 分子量：
- 230.11 g/mol
- 分子式：
- C7H3FN2O6
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Background: Members of the Rho family are essential regulatory components of the signaling pathway that direct cell motility, adhesion, and cytokinesis through reorganization of actin cytoskeleton. Rho is activated by extracellular signals such as lysophosphatidic acid (LPA). The actions of Rho are mediated by downstream Rho effectors. One of these effectors is Rho-associated kinase (ROCK). Two ROCK isoforms have been identified: ROCK-I (also known as ROKbeta) and ROCK-II (also known as Rho Kinase and ROKalpha). ROCK mediates Rho signaling and reorganizes actin cytoskeleton through phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. For example, ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). ROCK Activity Immunoblot Kit utilizes recombinant MYPT1 as ROCK substrate. After incubating the substrate with ROCK samples (such as purified kinase, cell lysate or immunoprecipitate), the phosphorylated MYPT1 is detected by western blot analysis using an anti-phospho-MYPT1 (Thr696) (Figure 1). ROCK Activity Immunoblot Kit provides a simple and fast tool to monitor ROCK activity using its physiological substrate. The kit also includes active ROCK-II as a positive control. Each kit provides sufficient quantities to perform 20 assays.
