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PEA-15
PEA-15
- 中文名称：
- PEA-15
- 英文名称：
- PEA-15
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA76638
- 规格：
- 0.1 mL|2x0.1 mL|3x0.1 mL|4x0.1 mL|5x0.1 mL
- 保存建议：
- Antibody can also be aliquotted and stored frozen at -20 degree C in a manual defrost freezer for six months without detectable loss of activity. The antibody is stableat 2-8 degree C for 1 year. Avoid repeated freeze-thaw cycles.
- 货期：
- 6-8周
- 来源宿主：
- Mouse
- 反应种属：
- Human, Mouse, Rat, Bovine
- 应用：
- IF (Immunofluorescence), WB (Western Blot), ICC (Immunocytochemistry)
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 其他：
克隆性:Monoclonal
同型:Mouse IgG
克隆号:N/A
特异性:N/A
纯度:N/A
形式：Affinity Purified antibody
Liquid. Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3
浓度：N/A
- 说明书：
PEA-15 was originally isolated as a major low molecular weight of embryonic mouse striatal astrocytes grown in cell culture. Three spots on 2D gels with an apparent molecular weight of 15kDa and isoelectric point 5.1-5.3 were shown to be different forms of one protein. The protein was serine phosphorylated on one site by protein kinase C both in vivo and in vitro and the protein was named "phosphoprotein enriched in astrocytes of 15kDa", hence PEA-15. Subsequent cloning and sequencing revealed a protein well conserved in sequence between mouse and human and which was heavily expressed in brain. Independently the same protein was found to be upregulated in fibroblasts and tissues of diabetic patients, and has hence named "protein enriched in diabetes" or PED. Immunocytochemical studies showed that the protein was heavily expressed in astrocytes and certain neurons in the CNS of mice, though it is expressed a lower levels ubiquitously. PEA-15 was shown to interact with extracellular signal regulated kinase and regulate the nuclear entry of this protein, and several other important interactions with other proteins involved in regulation of apoptosis, glucose metabolism and cell growth have been described. MCA-4D2 was made against a recombinant full length PEA-15 construct expressed in and purified from E Coli.
