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Anti-DTR/HBEGF Antibody Picoband
Anti-DTR/HBEGF Antibody Picoband
- 中文名称:
- Anti-DTR/HBEGF Antibody Picoband
- 英文名称:
- Anti-DTR/HBEGF Antibody Picoband
- 品牌:
- AAA Biotech
- 品牌介绍:
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具,核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号:
- AAA19433
- 规格:
- 0.01 mg|0.1 mg|0.1 mg (Carrier Free)|0.1 mg (HRP)|0.1 mg (FITC)
- 保存建议:
- Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months. Avoid repeated freezing and thawing.
- 货期:
- 6-8周
- 来源宿主:
- Rabbit
- 反应种属:
- Human, Mouse, Rat
- 应用:
- FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
- 免责声明:
- *本产品仅供科研实验使用,不得用于临床诊断。*
- 其他:
克隆性:Polyclonal
同型:Rabbit IgG
克隆号:N/A
特异性:Natural and recombinant Mouse CHI3L1
纯度:Immunogen affinity purified.
形式:Lyophilized
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
浓度:Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
- 说明书:
Background: Chitinase-3-like protein 1, also called CHI3L1 or YKL-40 is a secreted glycoprotein that is approximately 40kDa in size that in humans is encoded by the CHI3L1 gene. This gene is mapped to 1q32.1. Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling.
Principle of the Assay: The Quick Picokine™ Mouse CHI3L1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse CHI3L1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit contains recombinant Mouse CHI3L1 with immunogen: Expression system for standard: NS0; Immunogen sequence: Y22-A381. To measure Mouse CHI3L1, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product is linearly proportional to Mouse CHI3L1 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse CHI3L1 in the sample.