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TP53 Antibody
TP53 Antibody
- 中文名称：
- TP53 Antibody
- 英文名称：
- TP53 Antibody
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA301607
- 规格：
- 0.05 mL|0.1 mL|2x0.1 mL|3x0.1 mL|4x0.1 mL
- 保存建议：
- 储存在-20摄氏度
- 货期：
- 6-8周
- 来源宿主：
- Rabbit
- 反应种属：
- Human
- 应用：
- IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 其他：
克隆性:Polyclonal
同型:N/A
克隆号:N/A
特异性:The antibody detects endogenous level of total TP53 protein.
纯度:Antibodies were purified by affinity purification using immunogen.
形式：Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
浓度：1.0 mg/ml
- 说明书：
The TP53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of TP53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). TP53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro (2,3). DNA damage induces phosphorylation of TP53 at Ser15 and Ser20 and leads to a reduced interaction between TP53 and its negative regulator, the oncoprotein MDM2 (4). MDM2 inhibits TP53 accumulation by targeting it for ubiquitination and proteasomal degradation (5,6). TP53 can be phosphorylated by ATM, ATR, and DNA-PK at Ser15 and Ser37. Phosphorylation impairs the ability of MDM2 to bind TP53, promoting both the accumulation and activation of TP53 in response to DNA damage (4,7). Chk2 and Chk1 can phosphorylate TP53 at Ser20, enhancing its tetramerization, stability, and activity (8,9). TP53 is phosphorylated at Ser392 in vivo (10,11) and by CAK in vitro (11). Phosphorylation of TP53 at Ser392 is increased in human tumors (12) and has been reported to influence the growth suppressor function, DNA binding, and transcriptional activation of TP53 (10,13,14). TP53 is phosphorylated at Ser6 and Ser9 by CK1d and CK1eta both in vitro and in vivo (13,15). Phosphorylation of TP53 at Ser46 regulates the ability of TP53 to induce apoptosis (16). Acetylation of TP53 is mediated by p300 and CBP acetyltransferases. Inhibition of deacetylation suppressing MDM2 from recruiting HDAC1 complex by p19 (ARF) stabilizes TP53. Acetylation appears to play a positive role in the accumulation of TP53 protein in stress response (17).
