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MOUSE ANTI BrdU
MOUSE ANTI BrdU
- 中文名称：
- MOUSE ANTI BrdU
- 英文名称：
- MOUSE ANTI BrdU
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA49682
- 规格：
- 0.02 mg|2x0.02 mg|3x0.02 mg|5x0.02 mg|4x0.02 mg
- 保存建议：
- This product is shipped at ambient tempeture. It is recommended to aliqout and store at -20°C on receipt. When thawed , aliquot the sample as needed. Keep aliquots at 2°C to 8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
- 货期：
- 6-8周
- 来源宿主：
- Mouse
- 反应种属：
- N/A
- 应用：
- ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry)
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 其他：
克隆性:Monoclonal
同型:IgG1
克隆号:[Bu20a]
特异性:N/A
纯度:N/A
形式：Purified
Purified IgG - Liquid
Phosphate buffered saline
浓度：IgG concentration 1.0mg/ml
- 说明书：
Mouse anti BrdU antibody, clone Bu20a recognizes the thymidine analogue bromodoxyuridine (BrdU), which can be incorporated into DNA during S-phase of the cell cycle. The BU20a antibody is suitable for detecting incorporated BrdU in a wide variety of cell types and is suitable for use on tissue sections in double-labeling techniques.
Specificity: BrdU; Mouse anti BrdU antibody, clone Bu20a recognizes bromodeoxyuridine (known as BrdU or BrdUrd). BrdU is a synthetic thymidine analog, which is incorporated to new DNA during replication instead of thymidine. BrdU can therefore be used to identify newly synthesized DNA. Mouse anti BrdU antibody, clone Bu20a, recognizes BrdU and other thymidine analogs; 5'-chloro-2'-deoxyuridine (CldU), 5'-iodo-2'-deoxyuridine (IdU) and 2'-deoxy-5-ethynyluridine (EdU), but only shows minimal reactivity with thymidine itself (Aten et al. 1992, Liboska et al. 2012, Magaud et al. 1989). Antibody detection of incorporated BrdU in cellular DNA is extensively referenced as an accurate method to monitor cell proliferation in vivo and in vitro. In cell proliferation assays BrdU staining is coupled with the use of a dye that binds total DNA such as propidium iodide (PI). BrdU can be administered diluted in the culture medium or, in vivo via intraperitoneal injection, subcutaneous osmotic pump implants (Tesfaiqzi et al. 2004) or in drinking water (Moser et al. 2004). BrdU can be used as a thymidine analog in a wide range of organisms ranging from mammalian cells, through reptiles and amphibians to invertebrate species and plants. Mouse anti BrdU antibody, clone Bu20a, is suitable for detecting incorporated BrdU in a wide variety of cell types and is suitable for use on tissue sections in double-labeling techniques (Makarev and Gorivodsky 2014).
