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5-Hydroxymethylcytosine (5-hmC) antibody (mAb)(Clone 59.1)
5-Hydroxymethylcytosine (5-hmC) antibody (mAb)(Clone 59.1)
- 中文名称：
- 5-Hydroxymethylcytosine (5-hmC) antibody (mAb)(Clone 59.1)
- 英文名称：
- 5-Hydroxymethylcytosine (5-hmC) antibody (mAb)(Clone 59.1)
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA59960
- 规格：
- 0.01 mg|0.05 mg|0.1 mg|2x0.1 mg|3x0.1 mg
- 保存建议：
- Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
- 货期：
- 6-8周
- 来源宿主：
- Mouse
- 反应种属：
- Human, Mouse
- 应用：
- IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), DB (Dot Blot)
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 其他：
克隆性:Monoclonal
同型:IgG2a
克隆号:[59.1]
特异性:N/A
纯度:Protein G Chromatography
形式：Purified IgG in 70mM Tris (pH8), 105mM NaCl, 31mM glycine, 0.07mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
浓度：1ug/ul
- 说明书：
Short Description: Our 5-Hydroxymethylcytosine (5-hmC) antibody (mAb)(Clone 59.1) was raised in a Mouse host. It has been validated for use in Dot blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry and Methyl DNA Immunoprecipitation; it has been shown to react with Human and Mouse samples, but the sequence is not species specific so it should react with a wide range of sample types.
Background: In addition to this monoclonal, We offers two polyclonal antibodies that recognize 5-hydroxymethylcytosine,a whole serum version and apurified IgG version .All are validated for use in methyl DNA immunoprecipitation (MeDIP). For customers that require the ability to quantitate the amount of IgG in the MeDIP reaction, we recommend either this monoclonal or the purified IgG polyclonal. The whole serum version is very high titre and should be used carefully (0.1-0.5 ul per IP) as not to generate high non-specific background. The whole serum version has been used successfully in immunofluorescence (IF, http://www.ncbi.nlm.nih.gov/pubmed/20639862, 2010), and the purified IgG version is likely to work in this application as well.
DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in aCpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. 5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates.
A second type of DNA methylation exists,5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into5-hydroxymethylcytosine by the TET family of cytosine oxygenases. This antibody was developed specifically to distinguish 5-hydroxymethylcytosine from 5-methylcytosine as conventional methods (enrichment by antibody or methyl DNA binding protein, enzymatic digestion and bisulfite sequencing) cannot do so.
It is possible that5-hydroxymethylcytosine (5-hmC) represents a pathway to demethylate DNA, as 5-hydroxymethylcytosine is repaired as mismatched DNA and replaced with unmethylated cytosine.
