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His-Tag (2A8) mAb
His-Tag (2A8) mAb
- 中文名称：
- His-Tag (2A8) mAb
- 英文名称：
- His-Tag (2A8) mAb
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA62297
- 规格：
- 0.05 mL|0.1 mL|0.2 mL|2x0.2 mL|3x0.2 mL
- 保存建议：
- Store at -20 degree C. Stable for one year from the date of shipment.
- 货期：
- 6-8周
- 来源宿主：
- Mouse
- 反应种属：
- All
- 应用：
- ELISA, IP (Immunoprecipitation), IF (Immunofluorescence), WB (Western Blot)
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 其他：
克隆性:Monoclonal
同型:IgG1
克隆号:N/A
特异性:His-Tag (2A8) Mouse mAb detects over-expressed or recombinant proteins containing the 6x His epitope tag.
纯度:N/A
形式：N/A
浓度：N/A
- 说明书：
Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. A system that has proven to be very successful relies on the insertion of a six histidine (His6) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni -chelating resins and purification by single step affinity chromatography. This polyhistidine sequence can then be removed by specific cleavage at sites recognized by enzymes such as thrombin or enterokinase, permitting the separation of the target protein from the polyhistidine tag. Visualization of such fusion proteins can be achieved by utilizing antibodies generated against specific peptide sequences downstream from the multiple cloning site.
