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Myosin IIA Heavy Chain (Ser-1943), phospho-specific Antibody
Myosin IIA Heavy Chain (Ser-1943), phospho-specific Antibody
- 中文名称：
- Myosin IIA Heavy Chain (Ser-1943), phospho-specific Antibody
- 英文名称：
- Myosin IIA Heavy Chain (Ser-1943), phospho-specific Antibody
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA71662
- 规格：
- 0.1 mL|2x0.1 mL|3x0.1 mL|4x0.1 mL|5x0.1 mL
- 保存建议：
- Store at -20 degree C. Stable for 1 year.
- 货期：
- 6-8周
- 来源宿主：
- Rabbit
- 反应种属：
- Human, Rat, Mouse, Chicken
- 应用：
- ICC (Immunocytochemistry), ELISA, WB (Western Blot)
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 其他：
克隆性:Polyclonal
同型:N/A
克隆号:N/A
特异性:This antibody was cross-adsorbed to unphosphorylatednMHC-IIA (Ser-1943) peptide before affinity purification using phospho-NMHC-IIA (Ser-1943) peptide (without carrier). The antibody detects a 200kDa* protein corresponding tonMHC-IIA on SDS-PAGE immunoblots of human A431, Jurkat, mouse C2C12, and rat PC12 cells. This reactivity is removed after treatment with lambda phosphatase. The antibody also detectsnMHC-IIA (Ser-1943) phosphorylation in chick Fibroblasts.
纯度:N/A
形式：Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
浓度：N/A
- 说明书：
Non-muscle myosin II is an actin-based motor protein essential to cell motility, cell division, migration, adhesion and polarity. This myosin forms a hexameric complex comprised of two heavy chains (NMHC-II), two essential light chains, and two regulatory light chains (RLC). In vertebrates, there are threenMHC-II isoforms (NMHC-IIA, nMHC-IIB, andnMHC-IIC), which exhibit distinct patterns of expression in cells and tissues. Regulation ofnMHC-II activity occurs through RLC and HC phosphorylation. RLCs are phosphorylated at Thr-18 and Ser-19, leading to activation of myosin II motor activity and increased myosin filament stability. By contrast, PKC phosphorylation of Ser-1/Ser-2 and Thr-9 in RLC may decrease activated myosin II interaction with actin.nMHC-II phosphorylation may be an important mode for regulating myosin-II assembly. PKC phosphorylatesnMHC-IIA on Ser-1916 in the C-terminal region andnMHC-IIB on multiple serines in the tailpiece. Casein kinase II phosphorylatesnMHC-IIA on Ser-1943 in the tailpiece and increases disassembly ofnMHC-IIA filaments.
