![Anti-CD123 [7G3], Mouse IgG2a](/skin/notimg.webp)
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Anti-CD123 [7G3], Mouse IgG2a
Anti-CD123 [7G3], Mouse IgG2a
- 中文名称：
- Anti-CD123 [7G3], Mouse IgG2a
- 英文名称：
- Anti-CD123 [7G3], Mouse IgG2a
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA72280
- 规格：
- 0.2 mg|2x0.2 mg|3x0.2 mg|4x0.2 mg|5x0.2 mg
- 保存建议：
- Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
- 货期：
- 6-8周
- 来源宿主：
- Mouse
- 反应种属：
- Human
- 应用：
- Blocking, FCM/FACS (Flow Cytometry), WB (Western Blot), IP (Immunoprecipitation), FCM/FACS (Flow Cytometry)
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 其他：
克隆性:N/A
同型:Mouse IgG2a, Kappa
克隆号:[7G3]
特异性:This antibody specifically binds to the IL-3R alpha-chain and completely abolishes its function. IL-3R is expressed on myeloid, lymphoid, and vascular endothelial cells, where it transduces IL-3-dependent signals leading to cell activation.
纯度:Protein A affinity purified
形式：PBS with 0.02% Proclin 300.
浓度：1mg/ml
- 说明书：
This antagonist antibody binds IL-3Ralpha and blocks IL-3 binding and IL-3-mediated functions. mAb 7G3 immunoprecipitated and recognized in western blots the IL-3R alpha chain expressed by transfected cells and bound to primary cells expressing IL-3Ralpha. The expression of IL-3Ralpha chain on various cells types was analysed by flow cytometry using this antibody (PMID: 8547680). It is reported that 7G3 inhibits IL-3Ralpha and betac dimerization (PMID: 8649415). This antibody was used in the study of neutrophils expressing IL-3 receptors which are activated by granulocyte-macrophage colony-stimulating factor or GM-CSF (PMID: 7579364). The original 7G3, mouse IgG2a antibody inhibited IL-3-mediated intracellular signaling of isolated acute myeloid leukemia (AML) CD34+ CD38- cells in vitro and reduced their survival (PMID: 19570512). Humanized version of this antibody CSL362 is available on request. CSL362, retains the ability to neutralise IL-3 and has enhanced affinity for the FcgammaRIIIa (CD16) on NK cells. In vitro studies have demonstrated that the increased affinity for CD16 correlates with greater antibody-dependent cell-mediated cytotoxicity (ADCC) against CD123 expressing cell lines (Busfield et al., 2012). CSL362 is being accessed for use in the treatment of myeloid leukemia (PMID: 24363400, 2524888, 26130514).
