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Goat anti Mouse C3c, conjugated with Biotin
Goat anti Mouse C3c, conjugated with Biotin
- 中文名称：
- Goat anti Mouse C3c, conjugated with Biotin
- 英文名称：
- Goat anti Mouse C3c, conjugated with Biotin
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA77665
- 规格：
- 1 mL|2x1 mL|3x1 mL|4x1 mL|5x1 mL
- 保存建议：
- The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 degree C; prolonged storage at or below -20 degree C. Itis reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozenand stored at or below -20 degree C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used toprepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should beavoided. Working dilutions should be stored at +4 degree C, not refrozen, and preferably used the same day. If a slight precipitationoccurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.Lyophilized at +4 degree C--at least 10 years. Reconstituted at or below -20 degree C--3-5 years. Reconstituted at +4 degree C--7 days
- 货期：
- 6-8周
- 来源宿主：
- Goat
- 反应种属：
- Mouse
- 应用：
- IB (Immunoblot), DB (Dot Blot), IHC (Immunohistochemistry), ICC (Immunocytochemistry), ELISA
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 其他：
克隆性:Polyclonal
同型:N/A
克隆号:N/A
特异性:Biotin-conjugated IgG fraction of polyclonal Goat antiSerum to C3c fragment of Mouse complement factor C3
纯度:N/A
形式：N/A
浓度：N/A
- 说明书：
In immunoelectrophoresis against fresh mouse serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c cab also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. The product does not react with any other proteins component of mouse serum or plasma. The biotin immunoconjugate to mouse C3c is used to determine the presence and pattern of C3 in tissue lesions using immunohistochemical staining techniques. Locally deposited immune complexes in tissue usually contain complement, pointing to activation of the classical pathway. Complement activation in vivo implies active disease and may contribute to the elicitation of the pathogenesis and he extent of tissue destruction. In ELISA and Western blotting to identify mouse C3c in serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:50 and 1:200; In ELISA and comparable nonprecipitating antibody-binding assays between 1:500 and 1:1,000.
