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Mouse anti double-stranded RNA (J2)
Mouse anti double-stranded RNA (J2)
- 中文名称：
- Mouse anti double-stranded RNA (J2)
- 英文名称：
- Mouse anti double-stranded RNA (J2)
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA77709
- 规格：
- 0.5 mg|2x0.5 mg|3x0.5 mg|4x0.5 mg|5x0.5 mg
- 保存建议：
- After reconstitution antibodies should be aliquoted and stored at -20°C or -70°C. After adding 10mM sodium azide undiluted antibody can also be stored at +4°C for a short period of time. For long term storage the mAb should be kept frozen. Repeated freezing/thawing cycles should be avoided.
- 货期：
- 6-8周
- 来源宿主：
- Mouse
- 反应种属：
- N/A
- 应用：
- IHC (Immunohistochemistry), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), ELISA, DB (Dot Blot)
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 其他：
克隆性:Monoclonal
同型:IgG2a kappa
克隆号:[J2]
特异性:N/A
纯度:Purity: Gel electrophoretically pure IgG antibody.
Purification Method: Affinity chromatography on Protein A-agarose.
形式：N/A
浓度：Concentration after reconstitution: 1.00mg/ml as determined by A280nm (A280nm = 1.47 corresponds to 1mg/ml antibody).
- 说明书：
Background: Mouse monoclonal antibody J2 recognizes double-stranded RNA (dsRNA) provided that the length of the helix is greater than or equal to 40 bp. dsRNA-recognition is independent of the sequence and nucleotide composition of the antigen. All naturally occurring dsRNAs investigated up to now (40-50 species) as well as poly(I).poly(C) and poly(A).poly(U) have been recognised by J2, although in some assays its affinity to poly(I).poly(C) is about 10 times lower than that to other dsRNA antigens. Over the past decade our double-stranded RNA (dsRNA) antibodies have been used extensively to detect and characterise plant and animal viruses with dsRNA genomes or intermediates. In addition, the anti-dsRNA antibodies can be used as a test tool to detect pathogens, including detection in paraffin-embedded fixed tissue samples (Richardson et al. 2010).The J2 anti-dsRNA IgG2a monoclonal antibody has become the gold standard in dsRNA detection. It was used initially for the study of plant viruses, but since the seminal paper of Weber et al. in 2006, where J2 was used to show that all the positive strand RNA viruses tested produced copious amounts of dsRNA in infected cells, this antibody has been used extensively in a wide range of systems, as documented in over 200 scientific publications.J2 can be used to detect dsRNA intermediates of viruses as diverse as Hepatitis C virus, Dengue virus, rhinovirus, Chikungunya virus, Rabies virus, Polio virus, Classic swine fever virus, Brome mosaic virus and many more in cultured cells and also in fixed paraffin-embedded histological samples.J2 has been used to elucidate how anti-viral responses are initiated, what counter-strategies viruses have adopted to avoid them, and to explore the viral life cylce by enabling ultrastructiural localisation studies of viral nucleic acid replication sites (Welsch et al., 2009 & Knoops et al., 2011).J2 has been used successfully in electron microscopy, in immunofluorescence microscopy, in immunohistochemistry, and various immunocapture methods, such as dot blots and ELISA. J2 has also been recommended as a test tool to detect whether an unkown pathogen is bacterial or viral in nature (Richardson et al., 2010). Recently J2 has also been used to monitor the removal of dsRNA from in vitro synthethised mRNA preparations that may have potential use in gene therapy (Kariko et al., 2011).
