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Human beta-site APP-Cleaving Enzyme 1 ELISA Kit
Human beta-site APP-Cleaving Enzyme 1 ELISA Kit
- 中文名称：
- Human beta-site APP-Cleaving Enzyme 1 ELISA Kit
- 英文名称：
- Human beta-site APP-Cleaving Enzyme 1 ELISA Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA83356
- 规格：
- 48-Strip-Wells|96-Strip-Wells|2x96-Strip-Wells|5x96-Strip-Wells|10x96-Strip-Wells
- 保存建议：
- 将所有试剂储存在2-8摄氏度
- 货期：
- 6-8周
- 检测种属：
- Human
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Intended Use This bACE1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human bACE1. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY bACE1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-bACE1 antibody and an bACE1-HRP conjugate. The assay sample and buffer are incubated together with bACE1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the bACE1 concentration since bACE1 from samples and bACE1-HRP conjugate compete for the anti-bACE1 antibody binding site. Since the number of sites is limited, as more sites are occupied by bACE1 from the sample, fewer sites are left to bind bACE1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The bACE1 concentration in each sample is interpolated from this standard curve.
