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Human soluble Cluster of differentiation 40 ligand ELISA Kit
Human soluble Cluster of differentiation 40 ligand ELISA Kit
- 中文名称：
- Human soluble Cluster of differentiation 40 ligand ELISA Kit
- 英文名称：
- Human soluble Cluster of differentiation 40 ligand ELISA Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA83394
- 规格：
- 48-Strip-Wells|96-Strip-Wells|2x96-Strip-Wells|5x96-Strip-Wells|10x96-Strip-Wells
- 保存建议：
- 将所有试剂储存在2-8摄氏度
- 货期：
- 6-8周
- 检测种属：
- Human
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Intended Uses: This sCD40L ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human sCD40L.
Principle of the Assay: sCD40L ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-sCD40L antibody and an sCD40L-HRP conjugate. The assay sample and buffer are incubated together with sCD40L-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the sCD40L concentration since sCD40L from samples and sCD40L-HRP conjugate compete for the anti-sCD40L antibody binding site. Since the number of sites is limited, as more sites are occupied by sCD40L from the sample, fewer sites are left to bind sCD40L-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sCD40L concentration in each sample is interpolated from this standard curve.
