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Rabbit Transforming Growth Factor Alpha ELISA Kit
Rabbit Transforming Growth Factor Alpha ELISA Kit
- 中文名称：
- Rabbit Transforming Growth Factor Alpha ELISA Kit
- 英文名称：
- Rabbit Transforming Growth Factor Alpha ELISA Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA83416
- 规格：
- 48-Strip-Wells-(Competitive)|48-Strip-Wells-(Sandwich)|96-Strip-Wells-(Sandwich)|96-Strip-Wells-(Competitive)|2x96-Strip-Wells-(Competitive)
- 保存建议：
- 将所有试剂储存在2-8摄氏度
- 货期：
- 6-8周
- 检测种属：
- Rabbit
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Principle of the Assay: TGFa ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for TGFa. Standards or samples are then added to the microtiter plate wells and TGFa if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TGFa present in the sample, a standardized prepaRabbition of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for TGFa are added to each well to "sandwich" the TGFa immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substRabbite solutions are added to each well. The enzyme (HRP) and substRabbite are allowed to react over a short incubation period. Only those wells that contain TGFa and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substRabbite reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentRabbition of standards. The TGFa concentRabbition in each sample is interpolated from this standard curve.
