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Human Soluble Lectin like oxidized low density lipoprotein receptor 1 ELISA Kit
Human Soluble Lectin like oxidized low density lipoprotein receptor 1 ELISA Kit
- 中文名称：
- Human Soluble Lectin like oxidized low density lipoprotein receptor 1 ELISA Kit
- 英文名称：
- Human Soluble Lectin like oxidized low density lipoprotein receptor 1 ELISA Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA83461
- 规格：
- 48-Strip-Wells|96-Strip-Wells|2x96-Strip-Wells|5x96-Strip-Wells|10x96-Strip-Wells
- 保存建议：
- 将所有试剂储存在2-8摄氏度
- 货期：
- 6-8周
- 检测种属：
- Human
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
For Samples: Cell culture fluid, body fluid, tissue homogenate, serum and blood plasma
Intended Uses: This SLOX1 ELISA kit is intended for laboratory research use only. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of SLOX1 in the sample, this SLOX1 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus SLOX1 concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of SLOX1 utilizes a multiclonal anti-SLOX1 antibody and an SLOX1-HRP conjugate. The assay sample and buffer are incubated together with SLOX1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SLOX1 concentration since SLOX1 from samples and SLOX1-HRP conjugate compete for the anti-SLOX1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SLOX1 from the sample, fewer sites are left to bind SLOX1-HRP conjugate. Standards of known SLOX1 concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of SLOX1. The SLOX1 concentration in each sample is interpolated from this standard curve.
