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Human SerpinF2/Alpha-2-antiplasmin PicoKine ELISA Kit
Human SerpinF2/Alpha-2-antiplasmin PicoKine ELISA Kit
- 中文名称：
- Human SerpinF2/Alpha-2-antiplasmin PicoKine ELISA Kit
- 英文名称：
- Human SerpinF2/Alpha-2-antiplasmin PicoKine ELISA Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA125030
- 规格：
- 96-Strip-Wells|2x96-Strip-Wells|5x96-Strip-Wells
- 保存建议：
- 请按照说明书指示
- 货期：
- 6-8周
- 检测种属：
- Human
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Principle of the Assay: The Picokine Human SERPIND1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human SERPIND1 with a 96-well strip plate that is pre-coated with antibody specific for SERPIND1. The detection antibody is a biotinylated antibody specific for SERPIND1. The capture antibody is A monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. The kit contains recombinant Human SERPIND1 with immunogen: Expression system for standard: NSO, Immunogen sequence: G20-S499. The kit is analytically validated with ready to use reagents. To measure Human SERPIND1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human SERPIND1 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human SERPIND1 in the sample!!Background/Introduction: Heparin cofactor II (HCII), a protein encoded by the SERPIND1 gene, is a coagulation factor that inhibits IIa, and is a cofactor for heparin and dermatan sulfate. It is mapped to 22q11.21. This gene belongs to the serpin gene superfamily. Serpins play roles in many processes including inflammation, blood clotting, and cancer metastasis. Members of this family have highly conserved secondary structures with a reactive center loop that interacts with the protease active site to inhibit protease activity. This gene encodes a plasma serine protease that functions as a thrombin and chymotrypsin inhibitor. The protein is activated by heparin, dermatan sulfate, and glycosaminoglycans. Allelic variations in this gene are associated with heparin cofactor II deficiency.
