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Rat BMP-10 PicoKine ELISA Kit
Rat BMP-10 PicoKine ELISA Kit
- 中文名称：
- Rat BMP-10 PicoKine ELISA Kit
- 英文名称：
- Rat BMP-10 PicoKine ELISA Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA125054
- 规格：
- 96-Strip-Wells|2x96-Strip-Wells|5x96-Strip-Wells
- 保存建议：
- 请按照说明书指示
- 货期：
- 6-8周
- 检测种属：
- Rat
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Principle of the Assay: The Picokine Rat Bdnf Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Rat Bdnf with a 96-well strip plate that is pre-coated with antibody specific for Bdnf. The detection antibody is a biotinylated antibody specific for Bdnf. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Rat Bdnf with immunogen: Expression system for standard: sf21; Immunogen sequence: H129-R247. The kit is analytically validated with ready to use reagents. To measure Rat Bdnf, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Rat Bdnf in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat Bdnf in the sample!!Background/Introduction: Brain-derived neurotrophic factor(BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor(NGF). BDNF and neurotrophin-3(NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. BDNF and other neurotrophins are critically involved in long-term potentiation(LTP). BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic(5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury.
