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Human CD164 PicoKine ELISA Kit
Human CD164 PicoKine ELISA Kit
- 中文名称：
- Human CD164 PicoKine ELISA Kit
- 英文名称：
- Human CD164 PicoKine ELISA Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA125055
- 规格：
- 96-Strip-Wells|2x96-Strip-Wells|5x96-Strip-Wells
- 保存建议：
- 请按照说明书指示
- 货期：
- 6-8周
- 检测种属：
- Human
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Principle of the Assay: The Picokine Human CD14 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CD14 with a 96-well strip plate that is pre-coated with antibody specific for CD14. The detection antibody is a biotinylated antibody specific for CD14. The capture antibody is A monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. The kit contains recombinant Human CD14 with immunogen: Expression system for standard: CHO, Immunogen sequence: T20-C352. The kit is analytically validated with ready to use reagents. To measure Human CD14, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human CD14 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CD14 in the sample!!Background/Introduction: CD14, Cluster of differentiation 14, ingle-copy gene encoding 2 protein forms: a 50-to 55-kD glycosylphosphatidylinositol-anchored membrane protein(mCD14) and a monocyte or liver-derived soluble serum protein(sCD14) that lacks the anchor. By in situ hybridization and study of somatic cell hybrid DNA that the gene is located at bands 5q23-q31. CD14 acts as a co-receptor(along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide(LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein(LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.
