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Human Phospholipase B1, membrane-associated (PLB1) ELISA Kit
Human Phospholipase B1, membrane-associated (PLB1) ELISA Kit
- 中文名称：
- Human Phospholipase B1, membrane-associated (PLB1) ELISA Kit
- 英文名称：
- Human Phospholipase B1, membrane-associated (PLB1) ELISA Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA13202
- 规格：
- 48 Tests|96 Tests|2x96 Tests|3x96 Tests|4x96 Tests
- 保存建议：
- 储存在2-8摄氏度
- 货期：
- 6-8周
- 检测种属：
- Human
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
By Western blot analysis using an antibody against guinea pig phospholipase B (PLB), Maury et al. (2002) detected a 97-kD polypeptide in the soluble and membrane fractions of human epidermis that was enriched in the soluble fraction. Consistent with these results, they found that PLB activity was greatest in the soluble fraction.
Trypsin had no effect on the molecular mass or enzymatic activity, suggesting that the protein they detected was already in an active form.
Immunolocalization experiments revealed that PLB is expressed in the entire human epidermis with an accumulation in the dermoepidermis junction. In situ hybridization also indicated expression in the entire epidermis.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PLB1 in samples. An antibody specific for PLB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
