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Alanine Aminotransferase (ALT/GPT) Activity Fluorometric Assay Kit
Alanine Aminotransferase (ALT/GPT) Activity Fluorometric Assay Kit
- 中文名称：
- Alanine Aminotransferase (ALT/GPT) Activity Fluorometric Assay Kit
- 英文名称：
- Alanine Aminotransferase (ALT/GPT) Activity Fluorometric Assay Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA177774
- 规格：
- 48 Tests|96 Tests|2x96 Tests|3x96 Tests|4x96 Tests
- 保存建议：
- Store at -20 degree C. Valid for 12 months
- 货期：
- 6-8周
- 检测种属：
- N/A
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Intended Uses: This kit can be used to measure alanine aminotransferase (ALT/GPT) activity in animal tissue, serum (plasma) and other liquid samples.
Principle of the Assay: ALT catalyze the amino conversion reaction between alanine and alpha -ketoglutaric acid to produce pyruvic acid and glutamic acid. Under the action of pyruvate oxidase, pyruvic acid generates H2O2, which reacts with the non-fluorescent substance to form fluorescent substance under the action of peroxidase. The activity of ALT can be calculated by measuring the increase of fluorescence value at the excitation wavelength of 535 nm and the emission wavelength of 587 nm.
Background/Introduction: Alanine aminotransferase (ALT) is widely found in plasma and various tissues of the body, including liver, kidney, heart and skeletal muscle. ALT is an important pyridoxal phosphate dependent enzyme in the intermediate metabolism of glucose and protein. Clinically, the activity of serum alanine aminotransferase is often used as a marker for alcoholic liver disease, liver cirrhosis and acute viral hepatitis.
