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Free Fatty Acids (FFA) Fluorometric Assay Kit
Free Fatty Acids (FFA) Fluorometric Assay Kit
- 中文名称：
- Free Fatty Acids (FFA) Fluorometric Assay Kit
- 英文名称：
- Free Fatty Acids (FFA) Fluorometric Assay Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA177775
- 规格：
- 48 Tests|96 Tests|2x96 Tests|3x96 Tests|4x96 Tests
- 保存建议：
- Store at -20 degree C. Valid for 12 months
- 货期：
- 6-8周
- 检测种属：
- N/A
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Intended Uses: This kit can be used to measure the non-esterified free fatty acids (FFA) content in serum, plasma, animal tissue samples.
Principle of the Assay: Free fatty acids produce acyl coenzyme A in the presence of acyl synthase, which produces hydrogen peroxide in the presence of acyl oxidase. In the presence of the enzyme and probe, hydrogen peroxide react to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is directly proportional to the concentration of free fatty acids.
Background/Introduction: Free fatty acids, also known as non-esterified fatty acids, are derived from dietary or the metabolism of adipose tissue. In adipose tissue, hormonesensitive lipase (HSL) decomposes triglycerides to produce glyc erol and fatty acids. Circulating in the body with free fatty acids combined with plasma albumin, used as an energy source easily absorbed by muscles, brains, and other tissues and organs. FFA is not only the product of fat hydrolysis, but also the substrate of fat synthesis. The concentration of FFA is related to lipid metabolism, glucose metabolism and endocrine function.
