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ViraBind AAV Purification Mega Kit
ViraBind AAV Purification Mega Kit
- 中文名称：
- ViraBind AAV Purification Mega Kit
- 英文名称：
- ViraBind AAV Purification Mega Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA44624
- 规格：
- 2 Preps|10 Preps|5x2 Preps|2x10 Preps|3x10 Preps
- 保存建议：
- Store ViraBind AAV Reagent B, Purification Columns and Centrifugal Concentrators at room temperature and all other kit components at 4 degree C until their expiration dates.
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- N/A
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Background: The viral system includes vectors developed from retrovirus (RV), adenovirus (AdV), adeno-associated virus (AAV), lentivirus (LV), and herpes simplex virus (HSV). AAV belongs to the family of Parvoviridae, a group of viruses among the smallest of single-stranded and non-enveloped DNA viruses. There are eight different AAV serotypes reported to date. Recombinant AAV-2 is the most common serotype used in gene delivery, and can be produced at high titers with a helper virus or AAV Helper Free System. AAV-2 can infect both dividing and non-dividing cells and can be maintained in the human host cell, creating the potential for long-term gene transfer. Because AAV-2 is a naturally defective virus, requiring provision of several factors in trans for productive infection, it is considered the safest viral vector to use. Recently a new vector, AAV-DJ, was developed using DNA family shuffling to create a hybrid capsid from 8 different AAV serotypes, resulting in a vector with significantly higher in vitro infection rates across a variety of cells and tissues. Recombinant AAV-2 and AAV-DJ vectors can be purified by CsCl gradient ultracentrifugation, iodixanol discontinuous gradient ultracentrifugation, and high-performance liquid chromatography (HPLC). The most popular technique, CsCl ultracentrifugation, is time consuming process which may result in poor recovery and quality (nonviral protein contamination and a high ratio of genome copies versus infectious units). ViraBind AAV Purification Mega Kit does not involve ultracentrifugation. AAV-2 or AAV-DJ is first purified from viral supernatant with a single-step column, then further purified/concentrated with a centrifugal concentrator (see Assay Principle below). The entire procedure takes about 3 hours. Each preparation can handle up to 10 x 150 mm dishes, resulting in 100 muL of highly purified AAV-2 (Figure 1) or AAV-DJ with low ratio of genome copies versus infectious units. ViraBind AAV Purification Mega Kit provides an efficient system for quick purification of AAV-2 or AAV-DJ with high recovery (>60%). The highly purified and concentrated viruses can be used in primary cell infections and in vivo applications.
