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Nigericin
Nigericin
- 中文名称：
- Nigericin
- 英文名称：
- Nigericin
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA52750
- 规格：
- 0.5 umol|2x0.5 umol|3x0.5 umol|5x0.5 umol|4x0.5 umol
- 保存建议：
- Store at <-20°C.
Upon reconstitution in DMSO, store at -20°C.
Reconstituted Nigericin is stable up to 1 year at -20°C.
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- N/A
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
INTRODUCTION: Activation of the NLRP3 inflammasome in myeloid cells follows exposure to pathogen-associated molecular patterns (first signal) and ATP (second signal). This leads to oligomerization and assembly of a high molecular weight (~700 kDa) multimeric inflammasome complex, which leads to the conversion of pro-caspase-1 into the catalytically active form. Inflammatory caspases, such as caspase-1, or interleukin-converting enzyme (ICE), play a central role in innate immunity by recognizing cytosolic foreign danger signals and initiating a two-fold response. First, caspase-1 proteolytically converts the proforms of the two important pro-inflammatory cytokines, interleukin-1ß (IL-1ß) and interleukin-18 (IL-18), into their active forms, which are secreted. Second, caspase-1 or caspase-11 triggers a form of lytic, programmed cell death known as pyroptosis. Nigericin is a potent microbial toxin derived from Streptomyces hygroscopicus. It acts as a potassium ionophore, inducing a net decrease in intracellular levels of potassium, which is crucial for oligomerization of the NLRP3 inflammasome and activation of caspase-1. Nigericin requires signaling through pannexin-1 to induce caspase-1 activation and IL-1ß processing and release. It has been shown to generate a robust caspase-1 activation response in various cell lines, including Jurkat and THP-I cells.
