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Antibody Sandwich ELISA Development Kit
Antibody Sandwich ELISA Development Kit
- 中文名称：
- Antibody Sandwich ELISA Development Kit
- 英文名称：
- Antibody Sandwich ELISA Development Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA52751
- 规格：
- 1 Pack|2x1 Pack|3x1 Pack|4x1 Pack|
- 保存建议：
- 请按照说明书指示
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- N/A
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Background: The Antibody-Sandwich (AS) format ELISA may be the most useful of the immunosorbent assays for detecting antigen because it is both sensitive and robust. An Antibody-Sandwich format ELISA refers to an ELISA plate configuration whereby the analyte being quantified is bound, or "sandwiched" between two layers of antibodies (i.e. capture and detection antibody). In this ELISA format, the plate wells are coated with a monoclonal or affinity purified polyclonal antibody ("capture" antibody). Following the antibody coating step, plate wells are then washed and incubated with a blocking buffer to block the uncoated regions and stabilize the coated antibody. Next, in the capture incubation step, the coated antibody captures target analyte present in samples (see Figure 1). Afterwards, the plate is washed and bound antigen is incubated with the "up" antibody which is used to quantify the amount or concentration of target analyte in the samples. The up antibody may be a monoclonal or affinity purified polyclonal, and it may be directly labeled with enzyme used for detection, such as HRP (in this case, the up antibody and detection antibody are the same). Alternatively, HRP labeled secondary antibody (detection antibody) specific for the unlabeled up antibody may be used in a subsequent incubation step. After the incubation period has ended, ELISA plate wells are washed to remove all non-specifically bound HRP conjugate (detection antibody). The next step is to add a chromogenic HRP substrate, such as TMB, to the plate wells. TMB substrate will allow for the visualization and electronic quantitation of how much target analyte was present within the samples. In general, the more intense the TMB color present within a respective sample-well, the greater the amount of analyte present. To summarize, the basic AS ELISA format is capable of quantifying the amount or concentration of target analyte present within test samples. This Antibody-Sandwich ELISA Development Kit was created to help guide end users through the common steps and frequently encountered issues associated with building an AS ELISA. The guide describes assay performance milestones that must be achieved to enable progression through the various development stages of the analyte-specific sandwich format ELISA.
