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General Assay Diluent
General Assay Diluent
- 中文名称：
- General Assay Diluent
- 英文名称：
- General Assay Diluent
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA52763
- 规格：
- 100 mL|500 mL|1 L|2x1 L|3x1 L
- 保存建议：
- Store at 2-8°C. Store at room temperature for one week.
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- Clear to light yellow liquid
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
General Assay Diluent is formulated for testing serum, plasma, urine, and cell cul-ture samples in all sandwich ELISA formats. Use of an assay diluent helps minimize matrix complexity diff er-ences between the sample (e.g., serum, etc.) and the diluent used to generate the standard curve of the ELISA. Large diff erences between the sample and the standard diluent matrices will result in under-recovery of the target analyte present in sample wells. Complex and concentrated protein environments (the matrix) present in serum or plasma samples will greatly reduce the antigen-binding eff iciency of the plate-adsorbed antibodies, resulting in a gross underestimation of the amount of target analyte present in the test samples. General Assay Diluent helps equalize the antibody-binding eff iciencies between the standard curve and the sample wells.
To use, simply add 50-100 μL to every well of the ELISA plate, including all wells designated for standards, controls, and samples. Then add the standards, controls, and samples to the plate. Mammalian protein ad-ditives included in the formulation serve to reduce non-specific interac-tions between the sample matrix proteins and the plate surface, thereby minimizing background noise. General Assay Diluent also inhibits com-plement and thrombin activity present in serum and plasma samples. Incorporation of an antimicrobial agent allows for room temperature bench-top use and extensive storage stability at 2-8°C. This assay diluent formulation ensures a more accurate and consistent ELISA performance, regardless of sample type.
