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Alternative Block ELISA Blocking Buffer, Synthetic
Alternative Block ELISA Blocking Buffer, Synthetic
- 中文名称：
- Alternative Block ELISA Blocking Buffer, Synthetic
- 英文名称：
- Alternative Block ELISA Blocking Buffer, Synthetic
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA52764
- 规格：
- 100 mL|500 mL|1 L|2x1 L|3x1 L
- 保存建议：
- Store at 2-8°C for 24 months. Store at Room Temperature for 1 week.
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- N/A
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Alternative Block is a protein-free, detergent-free ELISA blocking buffer. This unique blocking buffer contains a heterogeneous mixture of proprietary blockers and synthetic stabilizers that block the uncoated regions of the plate without the use of conventional cross-reactive protein additives or detergents.
Alternative Block minimizes non-specific binding interactions and reduces background noise. It also stabilizes the coated protein during long-term storage by providing a microhydrated environment for improved retention of antigen epitope and antibody binding activity. Room temperature blocking of the plate and long-term refrigerated storage of dried plates are made possible by an antimicrobial component.
Alternative Block is suitable for use in most monoclonal and polyclonal antibody capture ELISA tests (also known as sandwich ELISAs) and peptide/protein antigen-down ELISAs. When preparing plates, the antibody or antigen is typically coated using 50-200 uL of coating solution per well. After coating, plates are washed to remove unbound proteins and then blocked using a larger volume of blocking buffer than was used for coating, such as 300 uL per well. This ensures that all uncoated regions inside the well are blocked. A 96-well plate blocked using this method will require 28.8 mL of blocking solution. Allow 10% extra blocking buffer to account for losses during pipetting.
