-
Recombinant PHF8 protein
Recombinant PHF8 protein
- 中文名称：
- Recombinant PHF8 protein
- 英文名称：
- Recombinant PHF8 protein
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA59682
- 规格：
- 0.02 mg|1 mg|2x1 mg|3x1 mg|4x1 mg
- 保存建议：
- Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- Recombinant PHF8 protein is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2mM TCEP.
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Short Description: Recombinant PHF8 (accession number NP_055922.1) was expressed in Sf9 and contains an N-terminal DYKDDDDK tag with a molecular weight of 121.1 kDa. PHF8 (PHD finger protein 8), also known as Lysine (K)-specific demethylase 7B (KDM7B) and JmjC domain-containing histone demethylation protein 1D-B (JHDM1DB), is a member of the JmjC-containing (Jumonji-C) class of histone demethylase proteins that are involved in the regulation of genome function through the removal of methyl groups from histones. PHF8 has two N-terminal domains, a PHD finger that binds trimethylated lysine 4 of histone H3 (H3K4me3) and a Jumonji domain that demethylates monomethylated H3 Lys9 (H3K9me1),Histone H3 dimethyl Lys9 (H3K9me2), Histone H3 dimethyl Lys27 (H3K27me2)(which are all modifications associated with transcriptional repression) and also Histone H3 dimethyl Lys36 (H3K36me2). PHF8 depletion in mammalian neuronal cells results in decreased expression of follistatin and increased methylation of H3K9 and H3K27. It has also been suggested to function in gene silencing during brain development. Recombinant PHF8 can be used for enzyme kinetics and inhibitor screening studies.
Background: PHF8 (PHD finger protein 8), also known as Lysine (K)-specific demethylase 7B (KDM7B) and JmjC domain-containing histone demethylation protein 1D-B (JHDM1DB), is a member of the JmjC-containing (Jumonji-C) class of histone demethylase proteins that are involved in the regulation of genome function through the removal of methyl groups from histones. PHF8 has two N-terminal domains, a PHD finger that binds trimethylated lysine 4 of histone H3 (H3K4me3) and a Jumonji domain that demethylates monomethylated H3 Lys9 (H3K9me1), Histone H3 dimethyl Lys9 (H3K9me2), Histone H3 dimethyl Lys27 (H3K27me2)(which are all modifications associated with transcriptional repression) and also Histone H3 dimethyl Lys36 (H3K36me2). PHF8 depletion in mammalian neuronal cells results in decreased expression of follistatin and increased methylation of H3K9 and H3K27. It has also been suggested to function in gene silencing during brain development.
