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Recombinant Mononucleosomes (H3.3)-biotinylated
Recombinant Mononucleosomes (H3.3)-biotinylated
- 中文名称：
- Recombinant Mononucleosomes (H3.3)-biotinylated
- 英文名称：
- Recombinant Mononucleosomes (H3.3)-biotinylated
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA59703
- 规格：
- 0.02 mg|2x0.02 mg|3x0.02 mg|4x0.02 mg|5x0.02 mg
- 保存建议：
- Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
- 货期：
- 6-8周
- 纯度：
- The recombinant protein is >95% pure by SDS-PAGE.
- 产品形式：
- Recombinant Mononucleosomes (H3.3)-biotinylated (20ug protein + 20ug DNA) is supplied in 10mM Tris-HCl, pH8.0, 1mM EDTA, 2mM DTT and 20% glycerol.
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Short Description: Our Recombinant Mononucleosomes (H3.3)-biotinylated consists of 5000 bp of DNA (plasmid) and two molecules each of histones H2A that includes amino acids 1-130 (end)(accession number NM_003512), H2B that includes amino acids 1-126 (end)(accession number NM_003518), H3.3 that includes amino acids 1-136 (end)(accession number NM_005324) with a C-terminal Biotin tag, and H4 that includes amino acids 1-103 (end)(accession number NM_003548). Recombinant Mononucleosomes (H3.3)-biotinylated was generated in E Coli and has an observed molecular weight of 110 kDa. Nucleosomes comprise the smallest subunit of chromatin; they consist of 147 bp of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). Therefore, nucleosomes are more physiologically relevant substrates than histones and histone-derived peptides for use in in vitro studies. More importantly, some histone methyltransferases are significantly more active, as well as specific, when using nucleosomal substrates in HMT assays, such as DOT1L and NSD family enzymes. Recombinant Mononucleosomes (H3.3)-biotinylated are for enzyme kinetics and inhibitor screening.
Background: In vivo, histones are wrapped around by DNA in chromatin. Therefore, nucleosomes are more physiologically relevant substrates than histones and histone-derived peptides for in vitro studies. More importantly, some histone methyltransferases are significantly more active, as well as specific, when using nucleosomal substrates in HMT assays, such as DOT1L and NSD family enzymes. Nucleosomes are also widely used in histone methyltransferase screening assays to identify small molecular inhibitors for drug discovery.
