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Muscarinic Acetylcholine Receptor M4 (CHRM4) ACTOne Stable Cell Line
Muscarinic Acetylcholine Receptor M4 (CHRM4) ACTOne Stable Cell Line
- 中文名称：
- Muscarinic Acetylcholine Receptor M4 (CHRM4) ACTOne Stable Cell Line
- 英文名称：
- Muscarinic Acetylcholine Receptor M4 (CHRM4) ACTOne Stable Cell Line
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA62077
- 规格：
- INQUIRE
- 保存建议：
- Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130 degree C, preferably in liquid nitrogen vapor, until ready for use.
Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Shipping Note: Product is available for shipment in the United States, Canada and European countries. Please inquire for shipment to other countries.
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- N/A
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Description: This cell line is a HEK-293-CNG cell line that expresses a recombinant human CHRM4. HEK-293-CNG cells express a modified CNG (Cyclic Nucleotide Gated) channel that opens in response to elevated intracellular cAMP levels and consequently result in ion flux and cell membrane depolarization.
Human CHRM4 ACTOneTM is a HEK-293 CNG cell line that expresses recombinant human CHRM4. HEK-293 CNG cells express a modified CNG (Cyclic Nucleotide Gated) channel that opens in response to elevated intracellular cAMP levels and consequently result in ion flux (often detectable by calcium-responsive dye) and cell membrane depolarization which can be easily measured with fluorescent Membrane Potential Dye. The assay allows both end-point and kinetic measurement of intracellular cAMP changes with a FLIPR, or a fluorescence microplate reader.
Introduction: The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. M4 receptor-deficient mice exhibit increased locomotor simulation in response to D1 agonists, amphetamine and cocaine. Neurotransmission in the striatum influences extrapyramidal motor control, thus alterations in M4 activity may contribute to conditions such as Parkinson's Disease.
