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Phosphodiesterase 4C (PDE4C) ACTOne Stable Cell (CHO cell)
Phosphodiesterase 4C (PDE4C) ACTOne Stable Cell (CHO cell)
- 中文名称：
- Phosphodiesterase 4C (PDE4C) ACTOne Stable Cell (CHO cell)
- 英文名称：
- Phosphodiesterase 4C (PDE4C) ACTOne Stable Cell (CHO cell)
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA62078
- 规格：
- INQUIRE
- 保存建议：
- Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130 degree C, preferably in liquid nitrogen vapor, until ready for use.
Low Survival Rate of Cells After Thawing: Cell vials could have thawed accidentally. Store cell vials in liquid nitrogen immediately after receiving and keep frozen at all time.
Leaving the vial at 37 degree C for too long during thawing will lower the survival rate. Place the vial at 37 degree C until cells are just thawed.
Handle the cells gently. Don't tap the vial or pipette the cells too many times before plating the cells.
Replace the medium four hours after thawing or when the cells have settled to remove DMSO.
Slow Growth Rate of Cells: Do not split cells before they have completely recovered from thawing and reach at least 50% confluence.
Do not dilute cells excessively while splitting.
Spit cells before they reach 80 - 90% confluence.
Use Trypsin-EDTA solution to dissociate cells
Cells may not be able to recover to an optimal stage if trypsin- free dissociation buffer is used.
Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Shipping Note: Product is available for shipment in the United States, Canada and European countries. Please inquire for shipment to other countries.
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- N/A
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Description: This cell line is a CHO-K1-CNG cell line that expresses a recombinant human PDE4C. CHO-K1-CNG cells express a modified CNG (Cyclic Nucleotide Gated) channel that opens in response to elevated intracellular cAMP levels and consequently results in ion flux and cell membrane depolarization.
Human PDE4C ACTOneTM is a CHO-K1 CNG cell line that expresses recombinant human PDE4C. CHO-K1 CNG cells express a modified CNG (Cyclic Nucleotide Gated) channel that opens in response to elevated intracellular cAMP levels and consequently result in ion flux and cell membrane depolarization which can be easily measured with fluorescent Membrane Potential Dye. The assay allows both end-point and kinetic measurement of intracellular cAMP changes with a FLIPR, or a fluorescence microplate reader.
Introduction: The PDE4C receptor, also known as the Phosphodiesterase 4C receptor, is a member of the cyclic nucleotide phosphodiesterase (PDE) family, and PDE4 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes.
