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SARS-CoV-2 Pseudoviral Particles, 222V
SARS-CoV-2 Pseudoviral Particles, 222V
- 中文名称：
- SARS-CoV-2 Pseudoviral Particles, 222V
- 英文名称：
- SARS-CoV-2 Pseudoviral Particles, 222V
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA62221
- 规格：
- 5 mL|2x5 mL|3x5 mL|4x5 mL|5x5 mL
- 保存建议：
- Ships on dry ice. Store at -70°C
Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Shipping Note: Product is available for shipment in the United States, Canada and European countries. Please inquire for shipment to other countries.
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- N/A
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
It has been known that SARS coronavirus 2 (SARS-CoV-2) uses human ACE2 as the entry receptor and human proteases as the entry activators. The virus surface spike (S) protein mediates SARS-CoV-2 entry into cells. To fulfill its function, SARS-CoV-2 spike binds to the receptor human ACE2 (hACE2) through its receptor-binding domain (RBD) and is proteolytically activated by human proteases.
Our SARS-CoV-2 Pseudoviral Particles are replication-deficient MLV pseudotyped with the SARS-CoV-2 spike protein. They also contain the ORF for firefly luciferase as a reporter. They establish a pseudovirus entry assay for SARS-CoV-2 as the S protein mediated cell entry can be conveniently measured via the luciferase reporter activity. This pseudovirus assay isolates the SARS-CoV-2 viral entry from other steps of the viral infection cycle.
A new SARS-CoV-2 strain with an amino acid change at position 222 from Ala to Val besides the change at position 614 from Asp to Gly in the viral S protein predominated over time in locales where it was found. To support the study of this new SARS-CoV-2 strain, we established the SARS-CoV-2-614G-A222V Pseudoviral Particles with the spike protein carrying the 222V and 614G genotype (Genbank Accession #QHD43416.1, p.222A->V, p.614D->G)
