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2019-nCoV Spike protein RBD (K417N, E484K, N501Y)
2019-nCoV Spike protein RBD (K417N, E484K, N501Y)
- 中文名称：
- 2019-nCoV Spike protein RBD (K417N, E484K, N501Y)
- 英文名称：
- 2019-nCoV Spike protein RBD (K417N, E484K, N501Y)
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA73185
- 规格：
- 0.01 mg|0.02 mg|0.05 mg|0.1 mg|2x0.1 mg
- 保存建议：
- Store product at –70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
- 货期：
- 6-8周
- 纯度：
- >90%
- 产品形式：
- Recombinant protein stored in 50mM sodium phosphate, pH 7.5, 300mM NaCl, 150mM imidazole.
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Recombinant viral protein expressed in CHO cells.
Recombinant 2019-nCoV Spike protein S1 subunit, RBD (K417N, E484K, N501Y) (319-541) was expressed in CHO cells using a C-terminal his tag. The gene accession number is MN908947.
The receptor binding domain (RBD) of the SARS-CoV-2 spike glycoprotein recognizes the host ACE2 receptor and is a major determinant of viral entry and neutralization (1). SARS-CoV-2 (501Y.V2 variant) with mutations at the key sites in RBD (K417N, E484K and N501Y) has severely affected regions of South Africa (2). MD simulation indicates that the combination of E484K, K417N and N501Y results in the highest degree of conformational modifications of RBD when bound to hACE2, compared to either E484K or N501Y alone. E484K and N501Y increase affinity of RBD for hACE2 and the charge switch due to E484K leads to the formation of favorable contacts. As new variants displace the first-wave virus, it is pivotal to evaluate their transmissibility, virulence and their possible tendency to escape antibody neutralization (3).
