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Phospho-ErbB 2 Positive Control
Phospho-ErbB 2 Positive Control
- 中文名称：
- Phospho-ErbB 2 Positive Control
- 英文名称：
- Phospho-ErbB 2 Positive Control
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA75977
- 规格：
- 5 Applications|2x5 Applications|3x5 Applications|4x5 Applications|5x5 Applications
- 保存建议：
- -20 degree C for long term storage
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- Western Blot Positive Control in SDS sam Ie buffer
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Description:
Western Blot Positive Control for Phospho Receptor tyrosine-protein kinase erB-2 (PHER2)
Overview:
The tyrosine kinase receptor erbB2, also known in humans as HER2, is a member of the epidermal growth factor receptor (EGFR or erbB1) family, which also includes erbB3 and erbB4 that are gene products of proto-oncogenes. Protocogenes play an important role in normal proliferation of cells. The interaction of proto-oncogenes with other tumor suppressor proteins (P53, P14ARF etc.) determine the normal or abnormal course of cell cycle and cell division leading to uncontrolled cell proliferation (1). HER2 is found on chromosome 17 and codes for a protein that serves as a cell surface receptor. These HER receptors determine the regulation of cell growth and differentiation in a complex manner. HER family receptors form homo- or hetero- dimers with other compounds. The activation of these homoheterodimmeric complexes by ligand binding leads to activation of intrinsic tyrosine kinase activity that triggers a cascade of signal transduction activity in cell. Some of these signals are transduced across the cell membranes in to cytoplasm and triggers the gene activation. There are at least two humanized recombinant monoclonal antibodies (Trastuzumab and pertuzumab) that target extra cellular regions of the HER2 tyrosine kinase receptor and have been used in human trials for the treatment of breast cancer (2).
Herceptin-2 is a 1342 amino acid protein (approximate MW 170-172 kDa) when analyzed on reduced SOS-PAGE gels. HER2 is over expressed in cells of many cancer types. ErbB receptors bind to a family of growth factors, termed neuregulins/heregulin (NRG/HRG), which comprise NRG-1, -2, -3, and -4 and include multiple variants. HER 2 is a receptor tyrosine kinase with intrinsic tyrosine kinase domain. The Phospho-Her2 antibody (MBS543807) is made against the phospho-Tyrosine containing peptide (aa 1127-1138) from rat HER2 protein.
The PHER2-selective antibodies were generated against unique antigenic sequences form HER2 protein that is not found in any other protein in the gene bank. The Phospho-HER2 antibodies were affinity purified over immobilized antigen based chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer.
