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Rat PlGF
Rat PlGF
- 中文名称：
- Rat PlGF
- 英文名称：
- Rat PlGF
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA79147
- 规格：
- 0.005 mg|2x0.005 mg|3x0.005 mg|4x0.005 mg|5x0.005 mg
- 保存建议：
- The lyophilized rat PIGF, though stable at room temperature, is best stored in working aliquots at -20 degree C to -70 degree C. Avoid repeated freeze and thaw cycles.
- 货期：
- 6-8周
- 纯度：
- > 95% by SDS-PAGE & silver stain
- 产品形式：
- Freeze dried; 25 mM Tris, pH 8.5
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Placenta growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family of growth factors. PlGF and VEGF share primary structural as well as limited amino acid sequence homology with the A and B chains of PDGF. All eight cysteine residues involved in intra and interchain disulfides are conserved among these growth factors. As a result of alternative splicing, three PlGF RNAs encoding monomeric human PlGF-1, PlGF-2 and PlGF-3 isoform precursors containing 149, 179 and 219 amino acid residues, respectively, have been described. In normal mouse and rat tissues, only one PlGF mRNA encoding the equivalent of human PlGF-2 has been identified. Rat PlGF shares about 60% amino acid identity with human PlGF-2. The gene for PlGF has been mapped to rat chromosome 6. PlGF binds with high affinity to Flt1, but not to Flk1/KDR.
However, little information regarding the expression pattern and cellular localization of PlGF mRNA in rat placenta during pregnancy is known. RT-PCR analysis shows that the expression level of PlGF mRNA increased as gestation advanced. Using in situ hybridization histochemistry, positive cells of PlGF mRNA were detected in chorionic villi, in the trophoblast and stroma cells surrounding the blood vessels within chorionic villi on day 13 and 15. The expression pattern of PlGF mRNA in rat placenta during pregnancy demonstrates that PlGF plays a functional role for placental growth and fetal development during mid-late pregnancy.
The full ORF of rat PlGF (Met1-Leu158) was cloned from total RNA of rat sinusoidal endothelial cells using standard protocols. The native protein expressed in insect cells starts with Ala24.
