Anti-ERK3/MAPK6 antibody (Internal) (STJ72939)-Anti-ERK3/MAPK6 antibody (Internal) (STJ72939)-一抗检测抗体

: Anti-ERK3/MAPK6 antibody (Internal) (STJ72939)

Anti-ERK3/MAPK6 antibody (Internal) (STJ72939)

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品牌介绍：: Stjohnslabs为生物技术、制药和学术界的生命科学家提供研究试剂盒、抗体和试剂的专业供应商。我们的平台为蛋白质和代谢物的检测、鉴定和定量提供了广泛的研究材料。

其他：: 克隆性：Polyclonal
浓度：0.5 mg/mL
稀释范围：Peptide ELISA: antibody detection limit dilution 1:128000.
WB: In transfected HEK293 transiently expressing Mouse ERK3/Mapk6 fused to GFP the band with expected size is intensified when co-expressed with MK5 as the stabilizer for ERK3. No bands are observed in transfected HEK293 with empty vector and the same band is observed using anti-GFP antibody. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration, 0.5-1µg/ml.
IHC: Paraffin embedded Human Heart. Recommended concentration: 10µg/ml.
IP: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced with MK5/ERK3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5ug EB11926. Western blots of the IP were labelled with rabbit anti-ERK3 serum (CST) or with mouse anti-MK5 (SC). An approx 100kDa of ERK3 is only precipitated from lysates of those KO MEFs that have been rescued by the ERK3/MK5 expression construct as described in as described in PMID: 22508986. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.