Anti-MK5/MAPKAPK5 antibody (Internal) (STJ72940)-Anti-MK5/MAPKAPK5 antibody (Internal) (STJ72940)-一抗检测抗体

: Anti-MK5/MAPKAPK5 antibody (Internal) (STJ72940)

Anti-MK5/MAPKAPK5 antibody (Internal) (STJ72940)

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品牌介绍：: Stjohnslabs为生物技术、制药和学术界的生命科学家提供研究试剂盒、抗体和试剂的专业供应商。我们的平台为蛋白质和代谢物的检测、鉴定和定量提供了广泛的研究材料。

其他：: 克隆性：Polyclonal
浓度：0.5 mg/mL
稀释范围：Peptide ELISA: antibody detection limit dilution 1:32000.
WB: In transfected HEK293 transiently expressing either Mouse MK5 fused to GFP or the similar Mouse MK2 fused to GFP; only the expected band was observed in the MK5 lane after EB11927 labeling. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration, 0.5-1µg/ml.
IHC: In paraffin embedded Human Kidney shows brushborder staining in the PCT. Recommended concentration, 4-8µg/ml.
IP: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced wiith Mk5/Erk3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5ug EB11927 or EB11928. Western blots of the IP were labelled with mouse anti-MK5 (SC) or with rabbit anti-ERK3 serum (CST). An approx 55kDa of MK5 is only precipitated from lysates of those KO MEFs that have been rescued by the ERK3/MK5 expression constructas described in PMID: 22508986. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.